Cost-Effective Sweetener Production

Recombinant microbial hosts for efficient production of steviol glycoside sweeteners like rebaudioside M. The hosts are engineered to express genes for key enzymes in the steviol biosynthesis pathway. These include genes for UDP-glucose synthesis, steviol glycosylation, and synthesis of precursor molecules like UTP.

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Continuous purification of steviol glycosides like rebaudioside D and M from crude stevia extracts using simulated moving bed chromatography without adding organic solvents. The process involves passing the extract through a series of adsorbent beds with water as the mobile phase to selectively retain impurities and enhance the steviol glycoside content. The method provides a cost-effective and scalable way to obtain purified steviol glycosides without using organic solvents.

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Process to make high purity acesulfame potassium sweetener by controlling pH during synthesis to reduce impurities. It involves forming a cyclic sulfur trioxide adduct, hydrolyzing it to form acesulfame-H, neutralizing to form crude acesulfame potassium, then treating to make finished acesulfame potassium with low impurity levels. Keeping the neutralization pH at or below 11 reduces degradation and impurity formation.

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Process for producing high purity acesulfame potassium. The process involves concentrating a crude acesulfame potassium solution containing acesulfame potassium and acetoacetamide to form an intermediate acesulfame potassium composition with low acetoacetamide. The intermediate composition is then separated to obtain a finished acesulfame potassium product with reduced acetoacetamide compared to the crude input.

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A process for producing high purity acesulfame potassium with reduced impurities like 5-chloro-acesulfame potassium that can be difficult to separate out. The process involves minimizing contact time between a solvent and cyclizing agent during the reaction to form a cyclic sulfur trioxide adduct. The adduct is then hydrolyzed and neutralized to produce high purity acesulfame potassium with less than 35 wppm 5-chloro-acesulfame potassium.

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Producing steviol glycosides, such as rebaudioside A, from recombinant cells expressing modified UDP-glycosyltransferase enzymes. The modified enzymes have mutations compared to the wild-type Stevia rebaudiana enzymes. Expressing the modified enzymes in cells to enable more efficient and scalable production of steviol glycosides as natural sweeteners compared to extracting from Stevia plants.

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Biocatalytic method for producing sweeteners and flavor enhancers like homoeriodictyol dihydrochalcone and hesperetin dihydrochalcone from plant compounds like phloretin. The method uses enzymes like oxidases, reductases, and methyltransferases to convert phloretin into the desired dihydrochalcones via steps like hydroxylation and methylation.

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A process for producing high purity acesulfame potassium with reduced levels of impurities like 5-chloro-acesulfame potassium. The process involves short contact times between the acetoacetamide salt and cyclizing agent to form a cyclic sulfur trioxide adduct, followed by hydrolyzing, neutralizing, and crystallizing steps to obtain the finished acesulfame potassium.

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Production of high purity acesulfame potassium compositions by controlling pH during neutralization to reduce impurities like acetoacetamide-N-sulfonic acid. The process involves hydrolyzing a sulfur trioxide adduct to form acesulfame-H, then neutralizing to form crude acesulfame potassium. By maintaining a pH below 11, impurity formation is minimized.

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Novel Stevia rebaudiana plant cultivars with traits like self-compatibility, high leaf yield, improved disease resistance, and increased steviol glycoside content. The plants are produced by controlled breeding using self-compatible varieties. The plants are processed to extract steviol glycosides for use as sweeteners and flavor enhancers in consumables.

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Processes for producing high purity acesulfame potassium compositions containing low amounts of impurities like acetoacetamide. The processes involve concentrating and separating steps to reduce impurity levels. The crude acesulfame composition is evaporated to form a concentrated intermediate composition with less water and impurities. This is crystallized and filtered to produce the finished acesulfame potassium composition containing reduced acetoacetamide.

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Recombinant microorganisms such as yeast or fungi that have been genetically modified to produce steviol glycosides like stevioside and rebaudioside in high yields. The modifications involve introducing genes encoding key enzymes from the stevia plant biosynthetic pathway into the microorganisms. This allows the microorganisms to synthesize the sweet compounds from inexpensive feedstocks, offering a more cost-effective and scalable way to produce steviol glycosides compared to extracting them from the stevia plant.

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Biocatalytic process for producing steviol glycosides like reb A and reb M from starting materials like glycerol, glucose, maltodextrin or existing steviol glycosides. The process involves using an enzyme-producing microorganism to convert the starting material into the desired steviol glycoside. The process can be optimized for yield and purity of the target glycoside. The microorganism can also be engineered to enhance specific glycoside conversions.

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Producing stevia sweeteners through recombinant biomanufacturing using genetically engineered cells expressing novel glycosyltransferase enzymes. The process involves modifying the enzymes that convert steviol glycosides in the stevia plant to enhance production of targeted sweeteners like rebaudioside A.

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Producing highly purified steviol glycoside sweeteners like reb A and reb M using biocatalysts. The process involves contacting a starting composition with microorganisms or enzymes that can catalyze the conversion of the starting materials to the target steviol glycoside. This allows efficient and scalable production of steviol glycosides from various organic substrates, providing a cost-effective alternative to extraction from Stevia plants.

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Methods for producing high quantities of Rebaudioside D and Rebaudioside M stevia sweeteners using recombinant techniques. The methods involve expressing specific biosynthetic genes in yeast or other cells to create an artificial biosynthetic pathway for these steviol glycosides. The recombinant cells can produce Rebaudioside D and Rebaudioside M at much higher yields than natural stevia plants. This allows commercial production of these steviol glycosides for use as sweeteners in foods and beverages.

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Cultivating cardoon plants to obtain inulin, biomass, seeds, oil, and protein flour. The plants are grown in clayey soil without significant fertilization. The above-ground parts are harvested in year 1 before flowering. From year 2 onward, the below-ground parts are harvested between flowering and senescence. The roots are cleaned, dried, and extracted to obtain inulin. The remaining biomass is pressed to extract more inulin. The harvested above-ground parts provide additional biomass and seeds/oil/protein. The process enables integrated production of valuable products from the cardoon plants.

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Biocatalytic process to make highly purified steviol glycosides like rebaudioside A from organic compounds like glucose using enzymes. A key enzyme is UDP-glycosyltransferase UGT76G1, which adds glucose units to form rebaudioside A. The process involves converting starting compounds to target steviol glycosides by contacting with biocatalysts like enzymes or microorganisms. The starting compounds can be steviol glycosides or other organic compounds. The process allows efficient and economical production of highly purified steviol glycoside sweeteners using biocatalysis.

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Genetically engineering yeasts, bacteria, or other cells to produce steviol glycosides like rebA, rebD, and rebM at higher levels. The cells are modified to overexpress or reduce expression of a protein that mediates steviol glycoside transport. This enhances or limits the movement of the glycosides out of the cells, making it easier to recover them for use as sweeteners.

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Enhancing production of steviol glycosides by modifying the transport of these sweet compounds out of cells. The modification involves overexpressing or reducing levels of a protein that mediates steviol glycoside transport across cell membranes. This can be done in recombinant cells used for steviol glycoside production.

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Creating food ingredients and other products from Stevia rebaudiana plant biomass, specifically the leaves and stems. This involves processing the biomass through steps like milling, bleaching, depolymerization, and disintegration to produce cellulose powders. These powders can then be used as natural sweeteners, flavor enhancers, and bulking agents in food and beverages.

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Biocatalytic conversion of steviol glycosides using UDP-glucosyltransferase enzymes to make highly purified steviol glycoside compositions. The starting material is a steviol glycoside like rubusoside, stevioside, or rebaudioside. The steviol glycoside is converted to a target glycoside like stevioside or rebaudioside A by adding UDP-glucose and UDP-glucosyltransferase enzymes. The enzyme adds glucose units to the substrate glycoside. UDP is optionally recycled to UDP-glucose. The target glycoside can be purified from the reaction mixture. This enables efficient production of highly pure steviol glycosides from lower purity starting materials using biocatalysis.

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Production of mogroside compounds like mogroside V, a natural sweetener from monk fruit, through recombinant host cells like bacteria. The host cells are engineered to express genes encoding enzymes involved in the mogroside biosynthesis pathway from monk fruit. This enables the host cells to convert precursor molecules into mogrosides.

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Producing steviol glycosides, including the highly desired RebM, in recombinant hosts like yeast or bacteria. This allows efficient, scalable production of these sweeteners without the need for extraction from the Stevia plant. The recombinant production involves engineering genes encoding enzymes involved in UDP-glucose biosynthesis and glycosylation into the host organism.

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A system and method for preparing xylitol in an efficient and automated way. The system integrates evaporation, crystallization, and centrifugation steps to streamline production and improve yield while reducing energy and raw material usage. The system uses multiple parallel evaporators, crystallizers, and centrifuges with valves to control the flow of xylitol liquid through the system. The method involves reusing unconverted paste and cleaning liquid to optimize efficiency.

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A continuous, solvent-free purification process for sweet steviol glycosides like Rebaudioside D and Rebaudioside M from stevia extract. The process uses a series of adsorbents and separation techniques like simulated moving bed chromatography, nanofiltration, and filtration to enrich and purify the glycosides while removing impurities. The purification is done using only water as the solvent, without any organic solvents.

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Recombinant host cells and methods for producing steviol glycosides through fermentation. The host cells are modified to overexpress or reduce levels of a protein that transports steviol glycosides. They can produce higher quantities of these sweeteners compared to natural sources like Stevia plants.

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Biotechnological production of rebaudioside sweeteners using genetic engineering. Genetically modified yeast cells are able to efficiently convert stevia leaf extract to high-value rebaudioside compounds like rebaudioside D (RebD) and rebaudioside M (RebM). The cells express modified versions of UDP-glycosyltransferase enzymes that can convert the starting rebaudioside A to the desired rebaudiosides D and M at high yields.

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Recombinant production of steviol glycosides such as rebaudioside A, B, D and M using modified transporter genes in yeast to increase production and allow transport of the steviol glycosides into the culture medium. The invention provides a recombinant host that synthesizes steviol glycosides and transports them out of the cell. The host has modified expression of transporter genes to increase export of the steviol glycosides.

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Purifying stevia extract to remove nitrogen compounds from fermentation by crystallizing steviol glycosides from an aqueous solution. Crystallizing stevia glycosides in water, instead of organic solvents like ethanol, reduces nitrogen impurities.

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Increasing production of steviol glycosides like rebaudioside A, D, and M in recombinant microorganisms like yeast by engineering enzymes in the steviol biosynthetic pathway. Specifically, modifying a hydroxylase enzyme called kaurenoic acid 13-hydroxylase (KAH) that converts a precursor compound to steviol. Mutating specific amino acids in KAH increases its activity, leading to higher yields of steviol and steviol glycosides when expressed in yeast.

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Efficient biocatalytic process for producing highly purified steviol glycosides like rebaudioside A and stevioside using biocatalysts like enzymes and microorganisms to convert starting materials like glucose or glycerol. The process involves contacting the starting material with the biocatalyst to convert it into the target steviol glycoside. The target glycoside can then be separated and purified from the starting material. This enables scalable and economical production of highly purified stevia sweeteners through biotransformation.

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A process for producing high purity acesulfame potassium sweetener that avoids formation of impurities like acetoacetamide-N-sulfonic acid during synthesis. The key steps are using pH control during neutralization to prevent degradation reactions that form impurities, and avoiding harsh conditions that stress the acesulfame potassium molecules.

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A process for producing high purity acesulfame potassium compositions with low acetoacetamide impurities. The process involves concentrating and separating steps to reduce the acetoacetamide content of crude acesulfame potassium. Evaporating the crude composition to reduce water content produces an intermediate composition with lower acetoacetamide. Crystallizing and filtering that intermediate forms the finished acesulfame potassium with even lower acetoacetamide.

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Recombinant production of steviol glycosides in microorganisms through gene modifications to increase steviol glycoside excretion into the culture medium. The modifications involve genes encoding sugar transporters, efflux transporters, and deletions of trafficking adapter genes in the production host cells. This allows increased production and export of steviol glycosides like RebA, RebB, RebD, and RebM from the modified cells.

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Manufacturing customized sweeteners and pharmaceuticals using partial melting and recrystallization. The process involves partially melting a crystallizable carrier like erythritol in water, then adding a heat or moisture sensitive active like a natural sweetener extract. The mixture is cooled and the water is evaporated under vacuum to recrystallize the carrier with the active incorporated. This allows customization of sweetness levels or drug dosages while preserving heat-sensitive actives.

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A sugar solution produced from rice and amylase that has a high sugar content and lower levels of soluble protein compared to conventional rice-based sugar solutions. This is achieved by using specific conditions of temperature (90-150°C) and time (1-3.5 minutes) to rapidly decompose the rice starch into sugar while minimizing the extraction of other components like protein. The resulting sugar solution is useful for making seasonings, foods, and drinks.

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Production of the natural high intensity sweeteners steviol glycosides using recombinant host cells expressing engineered UDP-glycosyltransferase enzymes that can transfer different sugar moieties onto steviol. The modified enzymes improve yield and efficiency of steviol glycoside production compared to wild-type plant enzymes.

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Recombinant production of steviol glycosides using modified microorganisms like yeast, fungi, and bacteria. The modifications involve introducing genes to produce precursors like UDP-glucose and enzymes to convert them into steviol glycosides like RebM. This enables high-yield and scalable production of specific steviol glycosides for use as sweeteners.

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A biocatalytic process for producing purified compositions of steviol glycosides, like rebaudioside AM, by contacting starting compositions containing organic substrates like glucose with microbial cells/enzymes to convert and form the target glycosides.

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Processes for producing high purity acesulfame potassium compositions with low levels of impurities like acetoacetamide. The processes involve treating a crude acesulfame potassium composition containing acesulfame potassium and acetoacetamide to form a finished acesulfame potassium composition. The treatment includes concentrating the crude composition by evaporation and then separating the concentrated acesulfame potassium to obtain a final purified product. The evaporation and separation steps are conducted at low temperatures below 90C and 35C respectively to minimize impurity formation.

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Producing glycosylated diterpenes like steviol glycosides using recombinant hosts like yeasts that have been genetically modified to produce the glycosylated diterpenes. The recombinant hosts are engineered to express specific UGT enzymes that catalyze the glycosylation reactions. The modified hosts can be fermented to produce the glycosylated diterpenes.

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A continuous process for purifying steviol glycosides from stevia extracts using simulated moving bed chromatography without using organic solvents. The process involves passing the crude stevia extract through a series of filtration and chromatography steps to enrich and purify the steviol glycosides. The final product is a purified steviol glycoside extract containing Rebaudioside A, stevioside, and Rebaudioside C.

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Process for producing high purity acesulfame potassium compositions with low levels of impurities like 5-chloro-acesulfame potassium. The process involves cooling the cyclizing agent composition before reacting it with the acetoacetamide salt. This reduces impurity formation compared to using higher temperatures. The cooled cyclizing agent composition is reacted with the acetoacetamide salt to form the cyclic sulfur trioxide adduct. The adduct is then hydrolyzed and neutralized to form the finished acesulfame potassium composition.

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Process for producing high purity acesulfame potassium with low levels of impurities like 5-chloro-acesulfame potassium. The process involves contacting a solvent and cyclizing agent to form a composition, reacting acetoacetamide with the cyclizing agent in the composition to form a cyclic sulfur trioxide adduct, and then hydrolyzing and neutralizing the adduct to form the finished acesulfame potassium. The key step is limiting the contact time between the solvent and cyclizing agent to less than 60 minutes. This reduces formation of chlorine-containing compounds that can chlorinate the acesulfame precursor.

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Processes for producing high purity acesulfame potassium compositions with low levels of acetoacetamide impurities. The processes involve treating crude acesulfame potassium compositions to form intermediate and finished acesulfame potassium compositions. The treatment involves specific temperature and residence time limits to reduce stress on the acesulfame potassium and prevent degradation. This prevents formation of acetoacetamide impurities.

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Process for producing high purity acesulfame potassium compositions with reduced impurities like acetoacetamide-N-sulfonic acid. The process involves neutralizing the hydrolyzed sulfur trioxide adduct at specific pH ranges to reduce stress on the acesulfame potassium and prevent degradation. This reduces impurity formation compared to conventional high pH neutralization. The crude acesulfame potassium composition can then be treated to further purify the acesulfame potassium.

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Recombinant host cells capable of producing steviol glycosides like RebD and RebM in high yields. The cells have modified expression of genes encoding sugar transporters and other enzymes involved in steviol glycoside biosynthesis. The modifications increase excretion of the desired steviol glycosides into the culture medium.

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Producing high purity acesulfame potassium compositions with reduced levels of impurities like 5-chloro-acesulfame potassium. The process involves cooling the cyclizing agent composition before reacting it with the acetoacetamide salt. This reduces impurity formation compared to using higher temperatures. The cooled cyclizing agent composition is reacted with the acetoacetamide salt to form the cyclic sulfur trioxide adduct. The adduct is then hydrolyzed and neutralized to form the finished acesulfame potassium composition.

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Process for producing high purity acesulfame potassium compositions with low levels of acetoacetamide impurities. The process involves concentrating the crude acesulfame potassium composition to form an intermediate composition with less water and acetoacetamide. The intermediate composition is then separated to form the finished acesulfame potassium composition with even lower acetoacetamide levels. The key steps are evaporating the crude composition at lower temperatures to reduce acesulfame degradation, and crystallizing the intermediate composition at lower temperatures to avoid impurity formation.

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