Probiotic Microencapsulation Techniques for Milk

Probiotic microcapsule that can be used in the preparation of an oral probiotic preparation. The microcapsule includes a core material, an emulsified lipid layer wrapping the core material, and a wall material wrapping the emulsified lipid layer.

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Active ingredient-embedded particles and preparation method for food products, particularly jams and yogurts, that combine the stability of encapsulated ingredients with the desirable processing characteristics of food products. The particles contain encapsulated probiotics, omega-3 fatty acids, and turmeric, which are protected from degradation through a proprietary encapsulation process. The encapsulation involves a double-layer emulsion wrapping process that maintains the active ingredient integrity during processing and storage. The encapsulated ingredients are then fermented in a controlled environment to produce the final product. The encapsulation method enables the preservation of the active ingredients during processing, while maintaining their beneficial properties.

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A carrier for targeted intestinal delivery of probiotics that selectively protects probiotics from the adverse environment of the stomach while enhancing their survival and bioavailability in the intestine. The carrier utilizes a novel film-forming solution that incorporates microalgae with potent antioxidant properties, specifically Spirulina platensis, Haematococcus pluvialis, Salina salina, and Chlorella, which effectively neutralizes reactive oxygen species. This solution forms a three-dimensional network structure that protects probiotics from stomach acid while maintaining their viability. The film can be prepared using low-ester pectin, gelatin, and glycerin, and can be used to load probiotics for targeted colonization in the intestine. The film exhibits superior ROS scavenging activity compared to conventional carriers, enabling the delivery of probiotics to the intestine while maintaining their therapeutic efficacy.

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Encapsulating probiotics in a plant protein matrix through a novel process that preserves probiotic viability. The method employs a calcium salt buffer at pH 6-7, allowing the plant protein matrix to polymerize at a weakly acidic pH, while maintaining probiotic integrity. The process involves a two-stage heating step followed by calcium salt curing, followed by microencapsulation through extrusion. The resulting microcapsules retain high viability of probiotics, especially when compared to conventional dairy-based encapsulation methods.

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A novel method for encapsulating probiotics in microcapsules that enhances their survival and therapeutic efficacy in the gastrointestinal tract. The microcapsules contain a water-blocking core material with a pH-dependent solubility profile, an anion-coated outer layer that protects the probiotics from acidic and alkaline environments, and a bioactive coating that maintains probiotic viability. The microcapsules are formulated with a specific combination of ingredients that optimize the probiotics' resistance to environmental stressors while maintaining their therapeutic activity. The encapsulation process preserves the probiotics' live cell viability and enhances their survival in the colon environment. This approach enables the delivery of probiotics with enhanced stability and therapeutic efficacy in the gut, particularly in pet food formulations where probiotics are often compromised by processing conditions.

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A probiotic preparation with enhanced immune activity that achieves colonization and proliferation in the small intestine through a novel encapsulation method. The preparation comprises a probiotic preparation with high immune activity comprising a probiotic preparation with encapsulated probiotics that are resistant to gastric acid and bile salts. The probiotics are encapsulated in a two-layer microcapsule structure that maintains their viability and activity in the small intestine environment, where bile salts are present. This encapsulation mechanism prevents the probiotics from being degraded by gastric acid and bile salts, allowing them to colonize and proliferate effectively in the small intestine.

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Probiotic microcapsules for improving intestinal flora and a preparation method thereof. The microcapsules contain a blend of specific strains of bifidobacterium and lactobacillus probiotics encapsulated in a sodium alginate solution. The microcapsules aim to provide a versatile probiotic product that can address intestinal issues in a comprehensive manner compared to single-strain probiotics. The encapsulation process involves suspending the probiotic strains in sterile saline, adding sodium alginate, and then dropping the suspension into a calcium chloride bath to form the microcapsules.

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Preparation method for bifidobacteria microcapsules that improves viability, stability, and bioavailability of bifidobacteria probiotics for health benefits. The method involves coating bifidobacteria cells with a protective layer of trehalose followed by a secondary coating of zein. This provides protection against environmental stresses like heat, oxygen, and acid. The microcapsules can be used in probiotic, prebiotic, and dietary fiber composite products to improve viability and effectiveness of bifidobacteria during storage, transport, and digestion.

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A rapid and efficient method to encapsulate probiotics using composite gels made from low-esterified pectin and sodium alginate. The method involves dissolving the pectin and alginate separately, mixing them, slowly adding it to a cross-linking agent solution, and allowing it to gel. This composite gel provides better protection and stability for encapsulated probiotics compared to using either pectin or alginate alone. The composite gel improves probiotic survival during storage and digestion, potentially enabling higher efficacy delivery of probiotics.

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Coated probiotics with enhanced stability through the use of milk-derived phospholipids and aloe vera gel as a novel coating agent. The coating agent comprises phospholipids from milk fat and aloe vera gel, which are combined in a specific ratio to enhance probiotic stability, particularly in acidic and digestive environments. The coating agent protects probiotics from degradation while maintaining their viability and functionality, particularly during cold and room temperature storage conditions.

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A probiotic microcapsule system for enhanced survival and delivery of live bacteria, particularly for probiotic strains like Lactobacillus salivarius. The system integrates a probiotic powder with a microcrystalline cellulose and starch matrix, encapsulated in a chitosan-based coating. The coating provides a protective barrier against environmental stressors, including gastric acid and bile salts, while maintaining the probiotic viability. The encapsulation process ensures controlled release of the probiotics, allowing them to interact with the host's beneficial microflora. This system addresses the challenges associated with probiotic survival in the gastrointestinal tract environment.

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A method for preserving the efficacy of Bifidobacterium lactis subsp. lactis microbial inoculum through controlled drying and encapsulation. The preparation involves creating an emulsion with the microorganism and a protective agent, followed by encapsulation in a biocompatible wall material. The emulsion is prepared by mixing the microorganism with the protective agent, and the wall material is formed through a controlled Maillard reaction process. The emulsion is then processed through micro-embedding and spray-drying to form the final product. This method preserves the microorganism's viability and functionality during storage, maintaining its ability to colonize the intestinal tract.

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Encapsulation of bioactive compounds in milk fat globules and oleosomes for targeted delivery through food systems. The encapsulation method utilizes naturally occurring milk fat globules and oleosomes as carriers, where the bioactive compounds can diffuse into the carrier structures. The encapsulation process involves mixing the bioactive compound with the carrier fractions, allowing diffusion through the carrier structures, and then stabilizing the encapsulated compound. This approach enables precise control over the compound's distribution within the carrier system, with the ability to achieve high encapsulation efficiency and stability across different food matrices.

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Microcapsule for improving the gut microbiota, which has improved tolerance to acids and improved viability compared to a commercially available product comprising a microorganism and protective matrix. The microcapsule includes a microorganism, a fluidizing gas, a coating dispersion, and one or more fatty alcohols.

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Preparing microcapsules containing Lactobacillus paracasei probiotics using bergamot polysaccharide as the wall material. The method involves extracting bergamot polysaccharides, compounding them with sodium alginate and starch, and then encapsulating Lactobacillus paracasei in this mixture. This provides a healthier alternative to traditional embedding materials like sodium alginate alone. The bergamot polysaccharide coating improves resistance to gastric acid and bile salts, allowing better survival of the probiotics through the digestive tract.

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Preparing probiotic microcapsules with improved survival in the gut by layering biopolymers and starch to protect the probiotics. The method involves coating the probiotics with a multilayer of oppositely charged biopolymers like chitosan and pectin. Then, these multilayer-coated probiotics are embedded inside microporous starch particles. The negatively charged outer layer of the probiotics adheres to the positively charged inner surface of the starch. This layering provides multiple barriers and prolongs gut transit time to enhance probiotic survival.

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Probiotic bead that can reduce the influence of adverse factors such as gastric acid, choline, bile salt and the like on probiotics, keep high activity of the probiotics and enable the probiotics to smoothly reach intestinal tracts. The bead is made of a core composed of probiotic bacteria mud and a first embedding agent layer embedded outside the core, a sclerotium, a second embedding agent, and a third embedding agent layer.

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A probiotic microcapsule for dairy products that combines natural buckwheat flour encapsulation with the beneficial properties of sea buckthorn. The microcapsules contain probiotic bacteria, buckwheat flour, salt, and emulsifying agents, and are produced through a novel encapsulation process using sea buckthorn meal. The microcapsules are then stabilized through calcium chloride treatment and frozen before lyophilization. This innovative approach provides enhanced probiotic survival and activity during food processing and storage, while maintaining the natural nutritional profile of buckwheat flour. The microcapsules contain a blend of probiotic bacteria, sea buckthorn meal, and salt, and are suitable for use in dairy products like ice cream, yogurt, and cheese.

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A novel probiotic delivery system that combines bifidobacteria with prebiotic fibers to enhance probiotic activity and survival in the gastrointestinal tract. The system comprises encapsulated probiotic microcapsules with a wall material comprising pectin and sodium alginate, where the probiotic core is comprised of Bifidobacterium longum subsp. infantis and Bifidobacterium animalis subsp. lactis strains. The probiotic microcapsules are prepared through a unique encapsulation process that protects the probiotic components while maintaining their viability. This formulation provides sustained release of beneficial microorganisms into the gut, enhancing their therapeutic effects while improving the stability of the probiotic delivery system.

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Colon-targeted probiotic microcapsules for enhanced survival in the colon environment. The microcapsules are prepared by creating a nuclear layer of probiotic suspension and prebiotic mixture within sodium alginate, followed by a protein-receiving solution. The process involves controlled addition of probiotic and prebiotic components to the sodium alginate solution, followed by a protein-rich solution. The microcapsules are then sterilized and formulated with bile salt solution to simulate the colon environment. This multi-layered encapsulation method enhances the survival of probiotics in the colon compared to traditional single-layer encapsulation methods.

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Preparation of probiotic microcapsules with improved survival and delivery compared to existing methods. The microcapsules are made by adsorbing probiotics into positively charged starch particles, followed by layer-by-layer adsorption of biopolymers. This multi-layered structure protects the probiotics during digestion and prolongs their time in the gut. The key innovation is using modified starch with internal positive charge to trap the probiotics, preventing loss during digestion. The biopolymers further coat and protect the microcapsules.

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A probiotic delivery system that enhances probiotic colonization through controlled release of probiotic particles in the gut. The system comprises a core structure, a lipid layer, and a superoxide layer. The core structure is a spherical probiotic particle, while the lipid layer is directly connected to the outer surface of the core. The superoxide layer comprises multiple spherical probiotic particles arranged in a concentric structure. This unique arrangement enables sustained release of probiotics through the gut wall, with the superoxide layer maintaining the probiotics' viability and preventing premature competition with intestinal flora.

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A probiotic microcapsule that effectively embeds large numbers of live probiotics while maintaining their viability and therapeutic effects. The microcapsules contain a sclerotium protective layer with embedded probiotics, a pectin and xanthan gum coating that completely covers the sclerotium layer, and an enteric coating layer. The sclerotium layer provides protection from stomach acid and digestive enzymes, while the pectin and xanthan gum coating ensures probiotic stability and prevents degradation. The enteric coating layer enables controlled release of probiotics in the gastrointestinal tract, where they exert beneficial effects. The microcapsules achieve enhanced stability and performance compared to traditional probiotic delivery systems.

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A probiotic preparation that combines the benefits of probiotics with enhanced stability and resistance to gastric acid and bile salts. The preparation involves encapsulating probiotic microorganisms in a matrix of porous dextrin, which forms a stable and protective microencapsulation. The probiotics are then formulated into a water-in-oil emulsion, which is further processed into granules. This innovative approach provides a probiotic product with superior stability, resistance to environmental stressors, and enhanced bioavailability compared to conventional freeze-dried probiotics.

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Preparation method for probiotic microcapsules using a combination of extrusion granulation and fluidized bed spray granulation. The method enables the production of denser, more stable probiotic microcapsules with enhanced survival rates in adverse environments, particularly in food products. The microcapsules contain probiotic bacteria embedded within a hydrophobic wall material, which provides superior protection against environmental factors. The method achieves this through a two-step process involving granulation followed by fluidized bed spray granulation, resulting in microcapsules with improved shelf life and functional efficacy.

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Probiotic microcapsule that can be applied to the fields of medicines, health care products and foods. The microcapsule is formed by taking a powdery or granular edible solid-phase component such as probiotics and taking a binder solution as a liquid-phase component, preparing a core material in a fluidized granulation mode, and then uniformly coating the core material with one or more layers of wall materials in a fluidized bed spray coating mode.

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A method for preparing microcapsules containing probiotics that enhances their release properties in the gastrointestinal tract. The method involves creating microcapsules through a controlled process that maintains the probiotic cells' viability and activity while preventing premature degradation. The microcapsules are prepared by mixing sodium alginate and modified starch in a specific ratio, then spraying the mixture into calcium chloride solution. The resulting microcapsules are washed and filtered to remove excess solution, and then treated with physiological saline to enhance their stability in the acidic environment of the stomach. This approach ensures the probiotics maintain their therapeutic effects while preventing premature release in the intestine.

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Probiotic microcapsules that selectively deliver probiotic bacteria to the colon through a novel double-layer encapsulation system. The system utilizes sporopollenin as the encapsulating wall material, which provides a stable and biologically active matrix for probiotic bacteria. The double-layer encapsulation design ensures both protection and controlled release of the probiotics, enabling targeted delivery to the colon while minimizing degradation in the stomach. This approach addresses the challenges of probiotic delivery through traditional single-layer encapsulation methods.

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A 2-compartment system for preserving microorganisms, particularly probiotics, in formulations that can be stored without water exposure. The system comprises a first compartment containing microcapsules with a water-insoluble shell, and a second compartment containing an aqueous organic acid solution. The microcapsules contain viable microorganisms in a non-aqueous composition within their core, while the aqueous acid solution provides preservative protection without interfering with microorganism viability. This compartmentalized approach enables long-term storage of microorganisms in formulations that would otherwise require water exposure, while maintaining their viability.

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Probiotic microcapsule for maintaining strain activity and preparation method thereof. The microcapsule has a double-layer embedding of natural materials around the probiotic core. The inner layer uses casein, malt extract, and xylooligosaccharide to stabilize the probiotics. The outer layer adds plant polysaccharides and pectin to form a protective shell. This dual encapsulation improves probiotic survival, stability, and release in the gut.

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Double-layer microcapsule with duplicate protection for unstable probiotics, from influence (such as light, temperature of external environment and undesirable element Degree, humidity, pH, bile etc.), it is effectively prevent the reduction of probiotic active during using and storing, keeps combination to greatest extent The stability of object, activity and effective field planting rate, and embedding efficiency is high, embedding effect is good. The composition includes: 14 parts of bifidobacterium longum, 14 parts of lactobacillus acidophilus, 5 parts of lactobacillus fermenti, 5 parts of Lactobacillus helveticus, secondary cheese cream bar 2-10 parts of bacterium, 2-10 parts of Lactobacillus rhamnosus, 2-10 parts of streptococcus thermophilus.

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Composite probiotic microcapsules for enhanced probiotic efficacy, stability, and intestinal health benefits. The microcapsules incorporate a biocompatible wall material comprising sodium alginate, konjac glucomannan, and calcium carbonate, while containing a synergistic probiotic core comprising Bifidobacterium, Lactobacillus casei, and Lactobacillus plantarum. This dual-component formulation combines the protective benefits of the wall material with the functional advantages of the probiotic core, enabling higher probiotic concentrations and improved intestinal health outcomes compared to traditional probiotic encapsulation methods.

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Probiotic combinations preparation for food products that can improve gut health and immunity. The preparation includes milk powder, sweetener, probiotic powder, and probiotic protective agent. The probiotic powder contains Lactobacillus rhamnosus LGG and animal bifidobacteria BB-12. The protective agent helps the probiotics survive the stomach acid and reach the gut. The combination of milk, sweetener, and probiotics provides a tasty and effective way to improve gut health.

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A fermented probiotic formula goat milk powder and a preparation method thereof, enabling the production of probiotic dairy products through powdering of fermented probiotic goat milk. The powdering process involves mixing goat milk powder with specific amounts of probiotic bacteria, stabilizers, and other ingredients to create a powder that can be easily administered directly into the body. This powder can be used to produce a wide range of probiotic dairy products, including fermented milk beverages and direct injection starter cultures, eliminating the need for liquid dairy products.

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A method for preparing probiotic microcapsule lyophilized powder through aqueous phase separation, enabling controlled preservation of probiotic viability during storage. The process involves stabilizing bacterial cultures in a skim milk solution, then coagulating the emulsion with CaCl2 at elevated temperature to form microcapsules. The resulting microcapsules are then lyophilized to preserve their probiotic content. The method ensures consistent bacterial viability and stability during storage conditions, enabling effective probiotic delivery in food products.

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A novel method for preparing lactic acid bacteria (LAB)-encapsulated probiotics through a multi-step process that enhances their survival and activity. The process involves activating LAB cultures, mixing them with sodium alginate and oxygen scavenger, and then encapsulating the mixture with chitosan. The chitosan layer forms a hydrophobic membrane that protects the alginate-algalacto-oligosaccharide complex while maintaining its charge balance. The encapsulated mixture is then treated with hydrochloric acid to create a stable membrane, followed by chitosan coating. This multi-layer encapsulation provides enhanced protection against gastric acid and bile, while maintaining the probiotic's viability and activity.

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Milk tablet that can be carried around, edible without heating without stored refrigerated. The tablet includes several active probiotics, and molding effect is preferable, flavor taste is preferable, the shelf-life is longer.

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Synbiotic microspheres comprising polyglucuronide and probiotics, formed through a novel emulsification process that leverages the unique properties of polyglucuronide to create stable, bioactive microspheres. These microspheres are engineered to incorporate probiotics directly into their matrix, enhancing their probiotic activity and bioavailability in the gut. The microspheres can be prepared through a controlled emulsification process that incorporates calcium chloride to facilitate microsphere formation and stabilization. This novel approach enables the creation of bioactive synbiotic microspheres with enhanced probiotic efficacy and sustained release characteristics.

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Microencapsulated probiotic bacteria that provide enhanced stability and antimicrobial protection through a novel encapsulation method. The probiotic bacteria are encapsulated in microcapsules made from a gelled alginate matrix, with an outer surface coated with a natural oil or treated with sodium chloride. The microcapsules protect the probiotic bacteria from environmental stressors like acidic solutions, high bile salts, and temperature extremes, maintaining their viability and activity for extended periods. The encapsulation method preserves the probiotic bacteria's metabolic activity and antimicrobial properties, enabling their application in food and beverage products.

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A method to enhance the probiotic delivery efficacy through encapsulation in microcapsules. The approach utilizes pectin and glucose as the encapsulation matrix and incorporates chlorine-coated calcium ions for improved probiotic protection. The probiotics are encapsulated within these microcapsules, which are then prepared using a controlled release process. The chlorine-coated calcium ions enhance the probiotics' resistance to acidic environments and digestive enzymes, while the pectin and glucose matrix provides a biocompatible and biodegradable barrier. This integrated approach enables targeted delivery of probiotics to the colon while maintaining their viability.

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Microencapsulated probiotic soft gelatin capsules with enhanced stability and viability at room temperature. The capsules contain encapsulated probiotics with a melting point between 35°C and 75°C between vegetable lipids, which maintains the probiotic's integrity and prevents agglomeration during storage. The encapsulation process involves suspending the probiotic formulations in a filler solution, followed by controlled mixing and deagglomeration. The resulting capsules maintain their probiotic viability and maintain a uniform particle size of 200 microns or less, enabling stable storage at room temperature for up to 24 months.

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Enhancing storage stability of probiotics through novel encapsulation methods. The invention utilizes semi-permeable microspheres containing probiotics, a high molecular weight polymer, and an effective bacteriostatic agent. These microspheres are introduced into food products with elevated humidity levels (10% or greater), allowing water to penetrate the encapsulation material. Refrigerated storage under controlled humidity conditions enables the probiotics to maintain viability, with activity reduced to 1.5 CFU/mL or less after six weeks. This approach addresses the conventional challenges of freeze-dried probiotics and extends their storage life beyond traditional limits.

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High-performance probiotic microcapsules for animal nutrition that enhance gut health and animal productivity. The microcapsules contain 95% probiotic bacteria, with a double-layer wall comprising sodium alginate and whey protein or gelatin. This advanced formulation protects the probiotics while maintaining their viability, enabling enhanced intestinal colonization and immune system support. The microcapsules can be prepared through fermentation of probiotic bacteria, with the fermentation medium optimized for maximum bacterial concentration and microcapsule formation.

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Prebiotic-enhanced probiotic microcapsules for enhanced gut health. The microcapsules are prepared by encapsulating probiotic bacteria in a biocompatible wall material matrix comprising sodium alginate and protein, with a specific ratio of 2:1. The wall material is formulated with soybean protein and lactalbumin at a concentration of 3-5%. The encapsulated probiotic bacteria are prepared through fermentation and subsequent sterilization, followed by centrifugation and sterilization of the bacterial suspension. The resulting microcapsules contain the probiotic bacteria in a stable, biologically active form.

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Microcapsules containing probiotics, prebiotics, and/or synbiotics that protect them from gastric conditions during transit through the stomach and intestinal environment, while releasing them upon exposure to intestinal conditions. The capsules are made from a biopolymer and plant-based protein, with enhanced protection against gastric conditions compared to conventional alginate-based capsules. The biopolymer acts as a matrix that maintains the probiotic cells within a controlled environment, while the protein enhances the capsule's structural integrity. The encapsulation process enables targeted delivery of the probiotics to the intestinal environment, where they can exert their beneficial effects.

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