Somatic Cell Count Determination for Milk Quality Assessment

A dairy system that optimizes somatic cell count measurement by selectively timing the test at specific intervals after milking, rather than conducting comprehensive daily counts. The system employs a rolling threshold approach, where somatic cell count is measured only after a predetermined time period following the initial milking session. This approach eliminates the need for daily somatic cell counts and reduces the number of tests required, particularly for animals showing early signs of mastitis.

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Determining somatic cells in raw milk through a novel analytical approach. The method employs a combination of optical and biochemical techniques to quantify the presence of somatic cells in milk. The analysis involves a combination of flow cytometry and biochemical assays to identify and quantify the specific cell types present, including epithelial cells from the mammary gland and leukocytes. This approach provides a comprehensive characterization of the somatic cell population in raw milk, enabling accurate monitoring of milk quality and disease incidence.

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A portable milk somatic cell counter and counting method for dairy products. The device comprises a fluorescence amplifying module, a disposable detection chip, and a mobile terminal with photographing capabilities. The detection chip contains a microfluidic system with a precisely optimized chamber height for single-cell counting, enabling accurate identification and quantification of somatic cells. The device integrates this technology with a user-friendly interface for on-site detection, eliminating the need for external equipment and reagents.

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Composition to reduce somatic cell count in milk and enhance milk yield through targeted bacterial intervention. The composition comprises Bacillus species specifically engineered to selectively target and reduce somatic cell populations in milk, while maintaining beneficial microbial populations. The engineered Bacillus strains exhibit enhanced growth rates and specific cell membrane modifications that enable their selective growth and proliferation in milk environments, while simultaneously inhibiting undesirable somatic cell populations. This targeted approach enables dairy farmers to maintain milk quality while reducing somatic cell counts to the established standard, thereby preventing mastitis-related losses.

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A method for detecting somatic cells in raw milk that enables accurate measurement without the need for cell activation. The method employs a specially designed immobilization chip that is manufactured by creating a collagen gel matrix. The raw milk is then spotted onto the gel matrix, and the immobilized cells are detected using a scanning electrochemical microscope. The measurement is based on the difference in oxygen reduction currents between the target cells and reference cells, eliminating the need for cell activation. The method maintains high measurement accuracy through the use of a cleaning pulse voltage to maintain electrode integrity.

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A method for accurately measuring somatic cells in raw milk through a novel staining technique that enhances resolution and reduces error. The method employs a specific staining protocol that targets the somatic cell-specific protein, lactoferrin, to improve detection sensitivity and specificity. This staining approach enables precise quantification of somatic cells in raw milk, overcoming limitations of traditional staining methods.

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HEK293 cell lines engineered to resist apoptosis and shear stress, enabling robust bioreactor culture and high-yield production of recombinant polypeptides and viral vectors. The engineered cells lack functional Bax and Bak proteins, which are essential for cell survival under stress conditions. The engineered cells can be used to produce therapeutic antibodies, antigens, enzymes, and vaccines, with improved productivity compared to conventional HEK293 cell lines.

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A milk somatic cell counter that enables rapid and accurate detection of milk somatic cells in raw milk through direct visualization of their morphology. The device employs a simple, automated process that uses a fluorescence-based staining protocol to selectively identify and count milk somatic cells. Unlike traditional methods that rely on surfactant-induced DNA release or flow cytometry, this approach directly stains and illuminates the cells, allowing for precise cell counting through optical detection. The device is particularly useful for monitoring milk quality and detecting mastitis in dairy farms, where accurate cell counting is critical for animal health management and milk quality assurance.

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Real-time somatic cell count monitoring system for dairy farms during milking, enabling early detection of mastitis through continuous cell analysis. The system integrates into the milking process, employing a portable, automated somatic cell counter that continuously measures cell counts at various levels along the milking line. This real-time monitoring enables early intervention before clinical mastitis develops, significantly reducing treatment costs and improving herd health. The system operates in parallel with the milking process, with the cell counter positioned between the milk source and the collective tank, allowing continuous testing throughout the milking cycle.

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Determining the precise number of somatic cells in milk through a quantitative analysis method that correlates with the diagnostic index of subclinical mastitis. The method enables accurate quantification of somatic cells in milk by employing a combination of process and equipment to provide precise and reliable results.

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A rapid detection system for milk somatic cells using fluorescence detection and circuit processing. The system employs a fluorescence detection module and a dedicated circuit system to rapidly quantify somatic cell counts in milk. The system includes a fluorescence detection module, a circuit system, and a control unit. The fluorescence detection module collects and processes the fluorescence signals from the detection module, which are then processed by the circuit system according to a predefined algorithm. The final results are displayed on a digital display.

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Determining the number of somatic cells in milk using a novel solution of Readep N, which enables precise enumeration of somatic cells in milk samples. The solution forms a distinct yellow-brown gelatinous mass when present in milk, with the number of cells corresponding to the gelatinous mass thickness. This method eliminates the limitations of traditional methods, particularly those relying on mastoprim solutions that have limited stability and accuracy. The solution's unique gelatinous mass formation characteristics allow for accurate enumeration of somatic cells in milk samples, particularly in compound milk, with high precision and reliability.

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A subclinical mastitis detection system that enables accurate and rapid detection of mastitis in dairy cows through automated milk sampling. The system integrates a laser-based optical scattering analysis system with automated milk sampling and processing capabilities. It uses a microfluidic chip technology to analyze milk composition and cell characteristics without requiring manual sampling, eliminating the need for traditional cell counting methods. The system can detect subclinical mastitis at concentrations as low as 200,000 cells/mL, providing real-time alerts for dairy farms to initiate treatment.

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A compact, automated milk somatic cell count measuring device for dairy farms. The device combines a milk somatic cell counter with an RFID reader, embedded development module, and a transmission module. The RFID reader connects to the development module, which is connected to the transmission module. The device measures milk somatic cells using the RFID reader and development module, while the development module records the cow's individual information and somatic cell count. The RFID reader and development module are connected via the transmission module, enabling real-time data transmission to the monitoring system.

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A single-chip microcomputer-based sensor for rapid detection of somatic cell counts in bovine milk, enabling low-cost, simple, and portable testing for dairy farmers. The sensor employs a low-cost, low-power microcontroller with specialized circuitry for impedance analysis, low-pass filtering, and analog-to-digital conversion. This compact solution enables rapid SCC measurement in raw milk, making it suitable for small-scale dairy operations and individual farmers.

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A feed for dairy animals that reduces somatic cell count (SCC) and total bacterial count in milk, achieved through lactic acid bacteria fermentation. The feed, produced by inoculating silage crops with lactic acid bacteria, achieves this through pH-dependent microbial inhibition. This feed enables dairy producers to maintain lower SCC and bacterial counts, significantly reducing the economic losses associated with mastitis and improving milk quality.

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Reducing milk somatic cell count in lactating ruminants by administering L-arginine or its analogues during gestation and postpartum periods. The supplementation increases plasma arginine levels, which in turn enhances the immune response of mammary gland cells, particularly neutrophils, to prevent mastitis infection. This approach enables sustained protection against mastitis while maintaining milk quality and quantity. The supplementation can be administered during the gestation period or postpartum, and the beneficial effects persist for up to 14 days post-administration.

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A method for automating cell enumeration and discrimination in raw milk using high-frequency impedance analysis in a microfluidic device. The method employs a microfluidic system that automatically calibrates and processes milk samples while simultaneously analyzing their impedance properties. The system determines the milk's impedance characteristics and calibrates the analysis parameters based on the inherent properties of milk components, particularly fat vesicles. This enables accurate cell counting and discrimination of cells from lipid vesicles and other non-cellular particles in raw milk, enabling more precise somatic cell enumeration and detection of pathogenic bacteria compared to traditional Coulter counters.

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