Electrophoresis Techniques for Plant Protein Extraction

A novel method for preparing highly functional plant proteins through membrane separation coupled physical field modification. The process involves membrane separation to obtain protein concentrate, followed by controlled alkaline treatment to enhance protein structure, and subsequent physical field modification using ultrasound and infrared to further improve protein properties. This method enables the preparation of proteins with enhanced solubility, foaming, and emulsifying capabilities, while maintaining their nutritional value and structural integrity.

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A triple-electrode electrophoresis device for enhanced extraction of biomolecules from large-volume samples. The device features a tank body with three annular electrodes: a central enrichment electrode, a ring enrichment electrode, and a ring collection electrode. The central and ring electrodes have the same polarity, while the ring electrode has the opposite polarity. This configuration creates a unique enrichment zone where biomolecules concentrate, while the ring collection electrode captures the enriched molecules. The device's tank body contains a collection system for the enriched biomolecules, allowing for efficient sample processing in medium to large-volume samples.

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Pulse protein isolate with enhanced functional properties, particularly in terms of texture and sensory characteristics, produced through a novel extraction and processing method. The isolate is characterized by its unique rheological behavior, with a linear viscoelastic region below 1000 Pa at 10% strain, and a density below 0.6 g/ml. The processing steps include extracting protein from milled pulse flour, pasteurizing the retentate fraction, and dewatering the resulting concentrate. The resulting isolate exhibits superior texture and sensory properties compared to conventional protein isolates, particularly in terms of crumb density, structure/texture, and mouthfeel.

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A device for efficient purification of charged DNA molecules from agarose gels, enabling direct separation and analysis without agarose. The device features a hollow channel with a porous dielectric membrane that allows electrophoretic migration while retaining charged species. The membrane is selectively permeable to the target analytes, enabling direct collection and analysis in solution. This approach eliminates the need for centrifugation or freezing, making it particularly suitable for high-throughput purification of DNA origami and nanoparticles.

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A method for extracting functional proteins from plant materials that retains their nutritional and functional properties. The method involves disrupting chloroplast membranes in plant juice, followed by selective precipitation of chloroplast membranes using a divalent ion. This selective precipitation process enables the removal of chloroplast membranes while preserving the soluble protein, resulting in a protein concentrate with minimal impurities. The method can be performed on a large scale and is particularly effective for obtaining functional proteins from plant materials like sugar beets.

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A process for producing high-purity protein extracts from plant materials, enabling the creation of stable and functional protein preparations for various food applications. The method employs a combination of enzymatic lysis and chemical treatment to generate a high-quality protein solution, followed by selective purification steps to achieve a purity of at least 80%. The process leverages plant-specific enzymatic activity to break down the plant material, followed by chemical treatment to remove contaminants. The purified protein is then further purified through selective adsorption, filtration, and ultrafiltration, resulting in a protein preparation with minimal impurities and excellent functional properties.

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An electrophoretic analyzer for analyzing organic materials through a novel approach that eliminates the need for gel transfer steps. The device integrates a buffer system into the electrophoresis chamber, allowing the separation of organic materials directly on the plate. The buffer system maintains optimal conditions for protein separation while preventing gel damage and maintaining membrane integrity. The device features a membrane-based transfer system, precision voltage control, and a circulation system to maintain optimal conditions throughout the analysis process. This eliminates the need for gel handling and transfer steps, reducing analysis time and complexity.

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A novel electrophoresis tank and column system for protein separation and detection that enables high-performance, high-throughput analysis of complex biological samples. The tank features a unique configuration with three buffer tanks positioned on the electrodes, allowing precise control of pH and ionic strength conditions. The tank is mounted on a column holder, which enables precise positioning of the column. The system incorporates a dedicated cooling system and elution solution delivery system, enabling rapid and efficient sample processing. The tank's design enables the simultaneous use of ICP-MS detection, making it an ideal platform for comprehensive protein analysis.

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Separating protein from Broussonetia papyrifera leaves using protein two-dimensional polyacrylamide gel electrophoresis. The method employs 2-DE to separate and analyze proteins from Broussonetia papyrifera leaves, providing a reliable and efficient method for studying plant proteome. The technique enables precise protein separation and identification, particularly challenging in plant-based protein separation applications.

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Electrophoresis cassette with reversible elution channels for improved separation efficiency. The cassette features a plate with multiple separation channels, including positive and negative elution channels, along with reversible elution modules that can be attached or detached. The reversible elution modules have adjustable positions within the plate's separation channels, allowing for precise control over elution conditions. This design enables optimized separation of analytes through both positive and negative elution channels, while maintaining reversible elution capabilities for continuous analysis.

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A device for efficient batch separation of protein mixtures through a single-step, planar electrophoresis process. The device employs a novel combination of ion-exchange and electrostatic interactions to achieve simultaneous separation of multiple protein components. The separation process is enabled by a unique combination of ion-exchange and electrostatic interactions between protein molecules and the device's surface, allowing for rapid and efficient separation of complex protein mixtures.

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A multi-row high-throughput horizontal continuous electrophoresis polyacrylamide gel system enables simultaneous detection of hundreds of samples in a conventional gel size range. The system employs a single gel plate with multiple parallel rows of polyacrylamide gels, each containing a uniform separation gel and an electrophoresis buffer. The gel buffer composition is optimized for high-throughput protein analysis while maintaining consistent migration characteristics across rows. The system achieves parallel detection of multiple samples simultaneously, enabling applications like protein quantification, parallel comparison of multiple samples, and large-scale screening.

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A protein separation and purification method that enables efficient and precise batch processing of complex protein mixtures through a single-step, continuous electrophoresis process. The method employs a device that combines multiple separation steps in a single run, allowing for simultaneous separation of multiple protein components based on their isoelectric point and molecular weight. This approach eliminates the need for multiple purification steps, reducing overall processing time and improving purity compared to traditional sequential purification methods.

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A two-dimensional electrophoresis system for Suaeda salsa that eliminates salt accumulation during sample preparation. The system employs a novel solvent extraction method that selectively removes salt ions from plant extracts without requiring specialized equipment. This approach enables efficient protein extraction from high-salt plant samples like Suaeda salsa, enabling reliable proteomic analysis.

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A plant cell protoplast purification instrument that simplifies the process of isolating and purifying plant cell protoplasts. The instrument features a unique funnel-shaped design with separate chambers for sample addition, cell debris collection, and protoplast collection. The protoplast collection chamber is positioned at the bottom of the instrument, with a filter separating it from the sample collection chamber. The protoplast collection chamber is connected to a positive electrode, while the cell debris collection chamber is connected to a negative electrode. The instrument also includes a protoplast collection plate positioned adjacent to the positive electrode, with a filter disposed above the protoplast collection tray. This configuration enables efficient protoplast collection while minimizing cell damage during the purification process.

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Optimizing protein extraction and two-dimensional electrophoresis for lily plants to produce high-quality protein profiles. The method employs a novel extraction protocol that improves protein yield and quality by optimizing the use of acetone, a common extraction solvent for plant proteins. This protocol overcomes common challenges in protein extraction, enabling the isolation of high-quality total protein from lily tissues. The extraction protocol is then followed by two-dimensional electrophoresis, where the extracted proteins are separated based on their isoelectric point and molecular weight. The resulting differential protein map provides detailed insights into the protein composition of lily tissues under stress conditions, enabling researchers to better understand the molecular mechanisms underlying disease resistance in lily plants.

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A device for precise protein classification and processing that enables efficient separation of plant proteins through a compact, single-stage system. The device features a rotating drum with multiple through holes, a motorized drum rotation system, and a series of rotating discs. The system uses a rotating drum to pass through the discs, with the discs rotating in synchronization to separate proteins based on size and composition. The separation process is optimized through precise control of the drum rotation speed and the positioning of the discs. The system includes a crushing press device, filtering device, and chromatography column, all integrated into a single frame that is mounted on a fixed frame.

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A novel method for extracting soluble leaf proteins from plant leaves for food and industrial applications. The process employs a buffer solution that maintains protein solubility throughout the extraction process, allowing the removal of non-protein components without protein denaturation. The buffer solution maintains optimal pH conditions for protein solubility while preventing protein aggregation. The method is scalable and can be adapted to various plant species, including tobacco, alfalfa, and spinach.

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Method for separating and purifying proteins from complex mixtures like cell extracts using two-dimensional gel electrophoresis (2-DE) without losing protein activity. The method involves running the first-dimension isoelectric focusing (IEF) gel without reducing agents to preserve disulfide bonds. For the second-dimension SDS-PAGE, the SDS concentration is reduced to 0.1%. This allows efficient separation of proteins while maintaining their enzymatic activity. After 2-DE, proteins are recovered from the gel using a specially designed protein elution plate with many small wells. This allows isolating individual proteins from the complex mixture. The plate has molecular semipermeable membranes that permit small molecules to pass through while retaining the proteins. This provides a simple, high-resolution method for isolating and purifying proteins from complex samples using 2-

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A two-dimensional gel electrophoresis method for separating proteins from complex biological samples, particularly for analysis of periplasmic proteins in Sargassum. The method employs gel eluted liquid fraction entrapment electrophoresis in the first dimension followed by isoelectric focusing in the second dimension. The separation is achieved through a novel approach where fractions are trapped in subsequent liquid phases, allowing precise resolution of proteins based on their charge. The method enables the separation of proteins differing in charge, including those with single charge changes, and is particularly suitable for analyzing proteins from complex biological sources like ribosomal assembly, plant extracts, and highly insoluble proteins like periplasmic proteins.

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