Ultra High Pressure Extraction of Plant Proteins

Drying non-mammalian proteins like plant proteins using a combined heating and drying process. The method involves atomizing and heating the protein liquid feed in a steam atmosphere at high pressures (5 barg or more) before direct drying in a spray dryer. This provides functionalized, texturized protein powders with reduced microbial loads and increased viscosity compared to conventional methods. The high pressure primary atomization and heating in the steam infusion chamber allows functionalization and concentration without dilution before drying.

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A method for extracting high-value active compounds from wheat sprouts using ultra-high pressure sub/supercritical fluid extraction. The extraction process involves grinding wheat sprouts into a fine powder, injecting the powder into a reactor, and using sub/supercritical fluid at ultra-high pressure to extract the desired compounds. The extracted compounds are then concentrated under reduced pressure to produce a high-purity extract. This approach enables efficient extraction of polyphenols, flavonoids, and other bioactive compounds from wheat sprouts, with enhanced yield compared to conventional extraction methods.

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Protein materials that can be presented in small capsules. The compaction of powdered protein materials can be carried out by applying high-hydrstatic pressure to reduce the water activity of the material.

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A process for producing high-purity protein preparations from plant biomass through enzymatic extraction. The method employs a buffer solution containing a reducing agent to extract proteins from plant materials, followed by mechanical lysis, enzymatic treatment, and separation steps. The extraction process maintains protein purity of at least 80% while achieving optimal protein recovery. The resulting protein preparations can be formulated into a wide range of food products with minimal impurities.

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A method for preparing plant-based protein powders that achieve superior water dispersibility and stability in drinking water. The method involves sequential extraction of plant material with water, followed by pH adjustment to 6-8, precipitation of the protein to form a solid precipitate, and subsequent high-temperature sterilization. The precipitate is then re-extracted with water to obtain a protein-rich solution, which is then sterilized at 100-180°C for 2-100 seconds. The sterilized solution is then re-extracted multiple times to achieve the desired protein concentration and water ratio. This process ensures the protein maintains its dispersion properties and stability in water, making it suitable for beverage applications.

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Process for preparing protein-fibre concentrates from plant materials like oilseeds, legumes and lentils that avoids denaturing the proteins during extraction. The process involves extracting water-soluble components from the plant material using mild conditions, then fractionating and concentrating the extract using declining polarity solvents. This replaces the initial aqueous extraction solvent with organic solvents. The final protein-fibre concentrate has high protein and fiber content, low carbohydrates, phenolics, and no long-chain organic or mineral solvents.

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Method for producing high-purity vegetable protein from wild hemp using ultra-high pressure technology. The method involves extracting protein from wild hemp, followed by ultra-high pressure processing to enhance protein quality and purity. This innovative approach leverages the natural resources of wild hemp while introducing cutting-edge ultra-high pressure technology to achieve superior protein yield and purity.

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A process for extracting high-quality protein isolates from plant materials like oilseeds, legumes, and lentils that contains high levels of oils, fats, and lipids. The process involves pre-treating the raw material to remove impurities, then extracting proteins using an aqueous solvent. The extract is then fractionated, concentrated, and purified using a sequence of declining polarity organic solvents. This allows isolating the proteins without co-extracting the oils and fats. The resulting isolates have high protein content, low carbohydrates, low phenolics, and no high-carbon solvents. They also retain native protein properties for food applications.

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A method for purifying proteins from plant biomass that maintains their native structure and properties. The purification process involves extracting the biomass with a solution containing polyethylene glycol (PEG) and an optional flocculant, followed by a two-phase separation step. The resulting PEG phase is collected and filtered to generate a protein solution. This solution is then incubated with PEG, allowing the protein to adsorb color and odor compounds. The PEG phase is then separated from the protein solution through gravity settling or centrifugation, and the resulting protein solution is further purified through ultrafiltration or other methods. The purified protein is then concentrated and sterilized to produce a final product concentrate.

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A novel approach to producing high-quality protein concentrates from forage crops like alfalfa that can be used as feed ingredients in animal nutrition. The concentrate is produced through a process that preserves the natural nutritional value of the forage while eliminating its undesirable anti-nutritional compounds. The process involves extracting and concentrating plant juice, then drying it directly to produce a protein-rich concentrate. This concentrate can be used as a feed ingredient in animal nutrition, particularly for livestock like pigs and poultry, while maintaining its nutritional value compared to traditional protein concentrates.

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