Reverse Micellar Extraction of Plant Proteins

A method for preparing plant-based protein isolate that enhances water dispersibility and stability through a two-step process. The process involves first extracting protein-rich liquid from plant materials at pH 6.9-7.5, followed by pH adjustment to the protein's isoelectric point ±0.1. The protein precipitates, and subsequent high-temperature sterilization at 100-180°C for 2-100 seconds preserves the precipitated protein while eliminating microbial contamination. The resulting protein product exhibits superior water dispersibility and stability compared to conventional methods, with optimal dispersion at pH 7.5 and 10 minutes holding time.

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A process for producing high-purity protein preparations from plant biomass through enzymatic extraction. The method employs a buffer solution containing a reducing agent to extract proteins from plant materials, followed by mechanical lysis, enzymatic treatment, and separation steps. The extraction process maintains protein purity of at least 80% while achieving optimal protein recovery. The resulting protein preparations can be formulated into a wide range of food products with minimal impurities.

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A process for extracting, purifying, and isolating proteins from plant materials like oilseeds, legumes, and lentils, with a focus on preserving protein structure and functionality. The process employs a novel combination of low-shear extraction and fractionation steps, followed by selective solvent replacement with ethyl acetate. This approach enables the efficient extraction of proteins from plant materials containing high amounts of lipids, while maintaining their native solubility and emulsifying properties. The resulting protein isolates retain their functional characteristics, making them suitable for use in food applications.

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A novel process for extracting protein-rich concentrates from plant materials with minimal solvent use. The method employs a combination of aqueous and organic solvents to extract soluble components from plant materials, followed by fractionation and purification using a selective precipitation step. The process achieves a protein content of at least 30% in the final concentrate, retaining the protein's native functional properties while minimizing the extraction of unwanted components like lipids and carbohydrates.

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A novel extraction medium for extracting lipophilic compounds from plant sources that enables selective and efficient extraction of desired agents. The medium is formed through a novel surfactant-based micellar system that spontaneously forms oil-in-water droplets upon dilution, encapsulating lipophilic compounds within its core. This formulation enables stable solubilization of lipophilic agents while maintaining their integrity at the interface between the oil and aqueous phases, allowing for controlled release upon dilution. The medium's composition can be tailored to optimize agent loading, selectivity, and yield through careful surfactant selection and formulation.

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A process for extracting high-quality protein isolates from plant materials like oilseeds, legumes, and lentils that contains high levels of oils, fats, and lipids. The process involves pre-treating the raw material to remove impurities, then extracting proteins using an aqueous solvent. The extract is then fractionated, concentrated, and purified using a sequence of declining polarity organic solvents. This allows isolating the proteins without co-extracting the oils and fats. The resulting isolates have high protein content, low carbohydrates, low phenolics, and no high-carbon solvents. They also retain native protein properties for food applications.

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A novel method for extracting plant proteins from raw materials that addresses the conventional problems of prolonged fermentation times, high water consumption, and environmental concerns. The process involves adjusting the pH of the raw material slurry to facilitate the precipitation of starch, followed by a controlled fermentation process that selectively separates protein, starch, and fiber components. The method eliminates the need for mechanical breakdown, sieving, and multiple precipitation steps, resulting in a more efficient and environmentally friendly extraction process.

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A novel approach to producing high-quality protein concentrates from forage crops like alfalfa that can be used as feed ingredients in animal nutrition. The concentrate is produced through a process that preserves the natural nutritional value of the forage while eliminating its undesirable anti-nutritional compounds. The process involves extracting and concentrating plant juice, then drying it directly to produce a protein-rich concentrate. This concentrate can be used as a feed ingredient in animal nutrition, particularly for livestock like pigs and poultry, while maintaining its nutritional value compared to traditional protein concentrates.

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