Creating meat-like textures from plant proteins requires precise control of protein denaturation, fiber alignment, and moisture distribution at the microscopic level. Current texturization methods achieve protein contents of 10-25% on a dry matter basis, but struggle to replicate the complex hierarchical structure of muscle tissue that gives meat its characteristic bite and mouthfeel.

The fundamental challenge lies in manipulating plant protein matrices to simultaneously achieve proper firmness, fiber orientation, and moisture retention while maintaining processability.

This page brings together solutions from recent research—including high-moisture extrusion with novel protein blends, enzymatic modification through protein deamidase, controlled gel network formation, and strategic use of cold-thickening polysaccharides. These and other approaches focus on creating consistent, scalable texturization methods that can be implemented in commercial production settings.

1. Method for Preparing Soy Protein-Based Emulsion Gel with Variable Texture Properties

JIANGNAN UNIVERSITY, 2025

A method for preparing an animal adipose tissue mimic using soy protein and its application, belonging to the technical field of deep processing of plant protein. The method involves preparing a soy protein/polysaccharide mixed emulsion gel that meets the requirements of mouth feel and texture and is healthier and more environmentally friendly, which not only meets the texture and melting property requirements of solid fat substitutes, but also provides technical support for the application of soybean protein/polysaccharide mixed emulsion gel in food fields such as meat products.

2. Raw Material Processing System with Shear Block Screw and Internal Heating for Pre-Extrusion Vegetable Protein Slurry Processing

INSTITUTE OF FOOD PROCESSING HEILONGJIANG ACADEMY OF AGRICULTURAL SCIENCES, 2025

Raw material processing system and method for producing high-moisture vegetable protein artificial meat that allows vegetable protein slurry to be extruded and heated before entering the extrusion machine. The system uses a screw with interspaced shear blocks and a heating assembly inside to fully process and heat the protein slurry. This prevents clumping and improves uniformity compared to feeding directly into the extrusion machine.

3. Plant-Based Meat Substitute with High Gel Strength Plant Proteins and Cold-Thickening Polysaccharides

BK GIULINI GMBH, 2025

Plant-based meat substitute that replaces animal-derived proteins and binders like methylcellulose with plant-based alternatives. The substitute contains a combination of high gel strength proteins and cold-thickening polysaccharides instead of methylcellulose. The high gel strength proteins are plant isolates like rubisco, potato, or canola that form self-supporting gels when heated. The cold-thickening polysaccharides like pregelatinized starch thicken at low temperatures. This allows the substitute to be shaped, heated to cook, and cooled without added binders like methylcellulose.

4. Plant-Based Meat Analogue with Rapeseed Protein Isolate for Enhanced Texture and Cooking Properties

DSM IP ASSETS BV, 2025

Meat analogue product made from plant proteins that closely mimics the texture and cooking experience of real meat. The product contains a texturized vegetable protein for structure, a binder, flavor, salt, and rapeseed protein isolate. The rapeseed protein isolate, containing cruciferins and napins, reduces weight loss during cooking, provides browning, and improves crust formation compared to using only texturized vegetable protein. The rapeseed protein isolate does not need to be texturized.

5. Vegetable Protein Meat Analog Comprising Soy Protein Isolate, Hemp Protein, Wheat Gluten, and Fatty Acids via High Moisture Extrusion

UNIV NORTHEAST AGRICULTURAL, 2024

Vegetable protein meat similar to chicken breast made by high moisture extrusion of soy protein isolate, hemp protein, and wheat gluten, along with fatty acids like lauric acid, myristic acid, and stearic acid. This improves texture, taste, and appearance compared to traditional vegetable proteins. The fatty acids increase protein network deformability, reduce density, and provide a more meat-like mouthfeel.

6. Method for Producing Plant-Based Meat Analogues Using Leuconostoc Carnosum Fermentation

CHR HANSEN AS, 2024

Producing meat analogue products from plant material using Leuconostoc carnosum to enhance flavor and texture. The process involves inoculating plant-based starting materials with Leuconostoc carnosum, which reduces volatile compounds like hexanal, pentanal, and furfural associated with off-flavors in these products. The inoculated materials are then processed through conventional extrusion or shear cell technology to produce meat analogues with improved flavor profiles and reduced spoilage potential.

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7. Heat-Coagulated Textured Fish Products with Integrated Plant-Based Protein

HAILIA NORDIC OY, 2024

Sustainable production of textured fish products by texturizing whole fish or fish parts with minimal processing. The method employs heat treatment to coagulate protein while preserving the natural texture and mouthfeel characteristics of the fish material. The resulting textured products achieve a protein content of 10-25% on a dry matter basis, with optimal texture and mouthfeel properties. The process can be combined with plant-based ingredients to enhance protein content, while maintaining the natural texture and flavor profile.

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8. Method for Incorporating Protein Deamidase in Plant Protein Mixture During Extrusion

NOVOZYMES AS, 2024

Method to improve the texture and water holding capacity of plant-based meat substitutes like burgers and sausages. The method involves adding a protein deamidase enzyme to the plant protein mixture before or during extrusion. This step deamidates glutamine residues in the protein, which improves the extruded protein's texture and water holding capacity when used in meat substitutes.

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9. Vegetarian Jerky Preparation Using Soy Protein and Alginate Gel Network Formation

HUZHOU ZHIRAN FOOD TECH CO LTD, 2024

Preparing vegetarian jerky with improved texture and flavor compared to traditional soy-based meat substitutes. The method involves mixing soy protein concentrate with sodium alginate at a specific ratio, then adding soy protein isolate and mixing again. The resulting mixture is extruded into jerky. The alginate from the concentrate forms a gel network with the isolate, improving the fiber and texture of the jerky. The final mass ratio of soy concentrate:alginate:isolate is 1:0.06:0.2.

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10. Textured Vegetable Protein Compositions with Enhanced Firmness via Dry Extrusion of High-Protein Vegetable Blends

ROQUETTE FRERES, 2024

Textured vegetable protein compositions made by dry extrusion that have higher firmness compared to existing textured pea proteins. The method involves dry extrusion of vegetable proteins like pea, oat, rice, or fava bean to create dense, fibrous textures similar to soy protein. The composition can have protein content above 80%. The dry extrusion process steps include blending, particle size reduction, extrusion, and drying. The extrusion conditions like pressure, temperature, and die size are optimized to maximize protein denaturation and fiber formation. The resulting textured vegetable protein can have higher density and firmness compared to textured soy protein.

11. Seaweed-Derived Protein Concentrate with Preserved Polysaccharide Structure via Novel Extraction Process

UMARO FOODS INC, 2024

Seaweed protein concentrate for plant-based meat alternatives, with enhanced taste, texture, and nutritional profile. The concentrate is derived from seaweed hydrocolloids like agar, carrageenan, and alginate, which are commonly used in food products. The concentrate is produced through a novel extraction process that preserves the natural polysaccharide structure of the seaweed while extracting the protein component. The resulting concentrate is formulated into a plant-based meat substitute that replicates the sensory attributes of traditional meat products, including flavor, texture, and color, while offering improved nutritional profile and environmental sustainability compared to conventional meat substitutes.

12. Recombinant Meat Protein Blend with Soy, Peanut, Wheat, and Collagen Proteins Forming Meat-Like Fiber Structure

NANJING CHUANGGUAN FOOD CO LTD, 2023

A recombinant meat protein made from a blend of soy, peanut, wheat, and collagen proteins that replicates the texture and flavor of meat when cooked. The protein blend is processed to create a structure similar to meat fibers when rehydrated. This involves cooking at lower temperatures and shorter times compared to traditional textured soy proteins. The resulting recombinant meat protein has a consistent meat-like texture when hydrated, unlike traditional textured soy proteins.

13. Plant-Based Food Composition with Texturized Vegetable Protein and Transglutaminase-Catalyzed Protein Crosslinking

AJINOMOTO KK, 2023

Plant-based food made with plant proteins, texturized vegetable protein, and transglutaminase enzyme. The plant protein-based food has 0.35-75% plant protein, 15-99% texturized vegetable protein, and 0.00015-0.30% transglutaminase. It provides a meat-like texture and mouthfeel without animal-derived binders like methylcellulose. The plant protein ingredients can be soy, pea, chickpea, etc. The transglutaminase catalyzes protein crosslinking.

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14. Composition of Textured Pea and Fava Bean Proteins with Adjustable Ratio and Firmness Comparable to Soy Protein

ROQUETTE FRERES, 2023

A specific composition containing a mixture of textured pea and fava bean proteins that provides similar firmness to textured soy proteins. The composition is made by cooking-extruding pea and fava bean isolates together. The pea:fava bean ratio can be adjusted. This mixture provides firmness equivalent to soy protein texturization, improving the texture of pea and fava bean protein products compared to isolates alone. The composition has water retention less than 2.5 g/g and firmness between 100-200 kg. It finds uses in food products like bakery, meat analogues, sauces, soups, and has applications in bakery-pastry, food, pharmacy, and cosmetics.

15. Two-Stage Extrusion Process for Plant Protein with Sequential Oil Enrichment and Fat Integration

Beijing Technology and Business University, BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY, 2023

A two-stage extrusion process for plant-based meat protein that enhances texture and appearance. The method involves first processing the plant protein to increase oil content, followed by a second stage of extrusion that incorporates the protein with added fats. This multi-stage approach significantly improves the protein's surface characteristics, texture, and overall mouthfeel, particularly in plant-based meat products that require a meat-like texture.

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16. Binding System for Vegetable-Based Meat Substitutes Using High Gel Strength Proteins and Cold-Thickening Polysaccharides

BK GIULINI GMBH, 2023

Label-friendly binding system for vegetable-based meat substitutes that replaces synthetic binders like methylcellulose. The system uses a combination of high gel strength proteins and cold-thickening polysaccharides to bind vegetable protein particles for making meat analogues. This allows shaping and processing the substitutes without synthetic additives. The proteins and polysaccharides provide viscosity, processability, and heating response similar to methylcellulose. The vegetable-based binding system enables making meat substitutes with cleaner labels by avoiding synthetic binders.

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17. Hybrid Meat Analogue with Aligned Protein Fibers via High Shear Mixing and Extrusion Cooking

UNIV MASSEY, 2023

Textureised hybrid meat analogue that improves the quality of food while minimising the adverse impact of meat consumption. The analogue comprises both plant and animal proteins and a process for preparing the same. The process involves using a high shear mixer and an extrusion cook to change the globular amorphous particles of protein into substantially aligned protein fibres.

18. Plant-Based Protein Meat Formation via Enzymatic Treatment and Wampee Bark Extract Integration

NORTHEAST AGRICULTURAL UNIVERSITY, 2023

Preparing plant-based protein meat that closely mimics the texture, taste, and appearance of real meat. The method involves enzymatically treating soy, pea, and gluten proteins to remove beany flavors. Then, extract from wampee bark is added to promote protein, water, and lipid binding. The mixture is extruded to form a fibrous structure. High-energy radiation sterilization completes the meat. This process creates a plant-based meat with natural texture, juiciness, hardness, elasticity, and animal flavors that closely resemble meat.

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19. Plant-Based Meat Analog with Crosslinked Pulse Proteins and Structured Vegetable Oils

CORN PRODUCTS DEV INC, 2023

Plant-based meat analogs that mimic the texture and mouthfeel of meat by loading vegetable oil into a water-soluble carrier and crosslinking pulse proteins like pea or fava bean. The analogs have structured vegetable oils with high oil-to-carrier ratios, pulse proteins with specific solubility and gelling properties, and moisture levels. Crosslinking pulse proteins using agents like transglutaminase or cysteine further improves texture. This allows making plant-based meats with better mouthfeel and texture compared to conventional plant protein gels.

20. Dry Extrusion Process for Textured Vegetable Protein from Rapeseed and Soy-Derived Proteins with Plant-Based Fibers

DSM IP ASSETS BV, 2022

A dry extrusion process for preparing textured vegetable protein (TVP) that overcomes common challenges in plant-based protein production. The process combines rapeseed protein with soy-derived protein and plant-based fibers to create a novel TVP composition. The composition is formulated to achieve optimal texture, flavor, and appearance characteristics for meat substitutes, particularly when processed through dry extrusion. The process enables the production of textured vegetable protein with improved mechanical properties, density, and texture profile compared to conventional plant-based protein sources. The composition can be used as a direct replacement for meat-derived protein sources in various food products, including meat substitutes, sausages, and processed meats.

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21. Plant-Based Meat Analog with Textured Pea Protein and Controlled Hydration Ratio

22. Cryogenic Treatment and Mechanical Shearing of Dehydrated Plant-Based Protein Fibers

23. Plant-Based Protein Matrix Utilizing Hemp Protein Isolates for Thermal Gelation and Extrusion

24. Meatball Production Process Incorporating Hydrolyzed Collagen and Textured Soy Protein

25. Method for Producing Plant-Based Sausages Using Soy Protein Rehydration in Oil-in-Water Emulsion

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