Rice Protein Extraction Techniques

A method for producing high-purity rice protein powder and rice syrup in an environmentally friendly way that improves protein quality and reduces cost compared to conventional methods. The process involves combining physical extraction with enzymatic extraction. It starts with homogenizing rice slurry at low temperature and pressure. The homogenized slurry is centrifuged to separate rice starch and protein. The starch-rich supernatant is further centrifuged to get the rice syrup. The protein precipitate is washed, filtered, and dried to get the high-purity rice protein powder. The wash water and filtrate are recycled to minimize waste.

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Extracting black rice bran protein with improved yield, purity, and color compared to existing methods. The extraction process involves enzymatic digestion, alkaline extraction, and hydrogen peroxide decolorization. The enzymatic step breaks down polysaccharides and anti-nutritional factors to release more protein. The alkaline step changes the protein charge to enhance solubility. The hydrogen peroxide step removes pigments for purer protein.

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A novel method for preparing low GI artificial rice that combines rice protein extraction with a novel rice protein blend. The method utilizes a specific rice protein extract that is prepared through a novel enzymatic process that enhances protein solubility and bioavailability. The extracted protein is then blended with a proprietary rice blend that combines traditional rice flour with a specially formulated rice protein concentrate. The resulting blend is then extruded into low GI artificial rice that maintains the characteristic texture and nutritional profile of traditional rice while addressing concerns related to protein content and texture.

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An optimized method for extracting and purifying yellow rice protein. The method involves degreasing the yellow rice flour, extracting the protein using an alkali-soluble acid precipitation, and further purifying the extract through gel column chromatography. The optimal conditions for each step are determined through experiments to maximize protein yield. This allows efficient extraction and purification of protein from yellow rice, which can be used as a nutritional supplement.

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Optimizing rice protein extraction through an enhanced alkali method that significantly increases protein yield. The method employs a controlled alkali treatment to enhance protein solubility, resulting in a higher protein recovery rate compared to conventional extraction methods. This approach enables the efficient extraction of rice protein from raw materials, reducing the environmental impact of wastewater treatment.

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Process to extract protein from rice grains with high yield and purity. The process involves treating the rice grains with alpha amylase to break down the starch, followed by alkaline protease to further hydrolyze the proteins. Then, ethanol and salt solutions are added to precipitate the protein, and finally, an alkali solution extracts the remaining protein. This multi-step process allows efficient extraction of protein from rice grains with improved solubility and purity compared to traditional methods.

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A method for making rice-based nutritional products from rice starch and rice protein through enzymatic hydrolysis. The process involves milling rice, preparing hydrated rice, mixing enzymes to form a starch-amylose complex, and then converting the complex into maltodextrin and protein fractions. The enzyme-treated rice is then processed through a combination of microfiltration and ultrafiltration membranes to separate the maltodextrin and protein fractions, followed by decanter separation to obtain the final product.

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A method for extracting rice protein from brown rice using alkaline extraction and salt precipitation. The method involves treating brown rice with an alkaline solution like sodium hydroxide to extract the protein, then precipitating the protein with salt like sodium chloride. This allows selective isolation of rice protein from the starch in brown rice. The extracted protein has improved solubility, emulsification, foaming, and gelation properties compared to conventional methods. The protein is also more concentrated and has higher water and oil retention.

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A green and efficient method for extracting and separating rice protein using a combined alkali and enzymatic extraction process. The method involves initially extracting rice protein using alkali at a low pH to partially hydrolyze the protein. Then, enzymes are added to further break down the protein into soluble peptides. This two-step process improves protein extraction efficiency compared to using alkali alone while avoiding the drawbacks of high alkali concentrations.

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A method to efficiently produce rice protein peptides with improved solubility, water retention, and foaming properties. The method involves super-fine grinding of rice residue protein after initial proteolysis to make it easier for enzymes to contact the substrate. This promotes proteolytic efficiency and taste during a second proteolysis step. The resulting rice protein peptides have improved properties for use in food, healthcare, beverages, cosmetics, etc.

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Extracting protein and starch from purple rice using fermentation with Lactobacillus paracasei. The method involves fermenting purple rice with Lactobacillus paracasei for 72-120 hours using sucrose as the carbon source and ammonium chloride as the nitrogen source. This results in high yields of both protein and starch from the rice. The fermentation conditions of temperature and time were optimized to maximize the extraction of protein and starch. The use of Lactobacillus paracasei and specific fermentation conditions allows efficient and selective extraction of protein and starch from purple rice without the need for harsh chemicals like alkali.

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Method for purifying high-purity black rice starch and soluble black rice protein from germinated black rice. The process involves using germinated black rice as a raw material and steps like micro-jetting, fermentation, enzymatic hydrolysis, cyclone washing, membrane filtration, and spray drying to separate and purify the starch and protein. This allows obtaining high-purity black rice starch with low protein content and soluble black rice protein rich in biologically active compounds like gamma-aminobutyric acid and flavonoids.

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A method to extract antioxidant protein from rice byproducts like rice dregs. The process involves soaking the rice dregs in water to extract the protein, then separating the protein through centrifugation and drying it to obtain high-purity rice protein powder. This allows utilization of low-protein rice byproducts like rice dregs to produce protein-rich rice protein powder with antioxidant properties.

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A method for efficiently extracting protein from broken rice that improves yield, reduces costs, and shortens extraction time compared to traditional methods. The process involves pulverizing, soaking, and preparing crude protein from broken rice to maximize protein extraction and reduce impurities. This allows more complete protein extraction from broken rice compared to conventional methods.

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A novel method for extracting high-quality rice protein through a multi-step process that leverages the unique properties of rice residue. The process involves adding water to rice dregs, adjusting the pH to optimize protein extraction, followed by centrifugation to separate the protein solution. The resulting protein solution is then treated with acid to adjust its isoelectric point, allowing precipitation of the protein. The precipitate is then dried to produce rice protein, which retains its nutritional value and functional properties.

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A method for rapid and efficient extraction of rice albumin from rice grains, followed by electrophoresis analysis to evaluate its quality. The extraction process involves grinding rice into flour, dissolving it in water, and then using centrifugation to separate the supernatant. The supernatant is then mixed with protein extracts and subjected to SDS-PAGE electrophoresis to separate and quantify the albumin protein. This approach enables the rapid identification of rice varieties with specific protein profiles, enabling early breeding selections for improved eating quality.

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Method to extract rice protein powder with higher protein content compared to traditional methods. The process involves soaking rice in a concentrated sodium hydroxide solution, followed by enzymatic and acid hydrolysis steps. The higher protein yield is achieved by using specific conditions during the initial rice slurry preparation step. The process involves soaking rice in a concentrated sodium hydroxide solution, followed by enzymatic and acid hydrolysis steps. The higher protein yield is achieved by using specific conditions during the initial rice slurry preparation step.

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A method for extracting high-quality rice protein powder from rice residue using a novel enzymatic pretreatment process. The method employs a specific combination of enzymatic hydrolysis and acid treatment to break down the complex glutenin and globulin structures in rice, followed by membrane filtration to separate the resulting peptide-rich protein. This enzymatic pretreatment step enables the efficient extraction of high-quality rice protein without the need for complex and costly protein extraction technologies.

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High-purity enzymolysis of rice protein through a multi-step process that improves protein quality and solubility. The process involves ultrafine pulverization of broken rice, acid-mediated deamidation to produce a crude protein, high-pressure treatment to enhance protein solubility, and complex enzyme treatment to further purify the protein. This multi-step approach addresses the limitations of traditional enzymolysis methods by addressing both protein quality and solubility issues.

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Rapid extraction of rice protein powder using vacuum negative pressure and rotary evaporation. The process involves rapidly precipitating rice protein from broken rice flour using negative pressure instead of soaking. This prevents deterioration due to heat or time. The slurry is then concentrated using rotary evaporation to extract the protein powder quickly. The process provides high protein purity and yield without environmental pollution.

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A method for extracting and processing rice protein from rice bran to produce high-quality protein concentrates. The process involves a single-step extraction of rice protein from rice bran using a combination of mechanical and enzymatic treatments. The extracted protein is then purified through a proprietary processing step that enhances its bioavailability and reduces allergenicity. The resulting protein concentrate is suitable for use in food products that require hypoallergenic ingredients, particularly in infant nutrition and allergy prevention applications.

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A method for selectively recovering high-quality protein from rice, particularly prolamin, with enhanced purity and yield. The process involves dissolving the protein in an alkaline-alcohol solution to solubilize the target protein, followed by neutralization of the supernatant with an acid to precipitate non-protein components. The purified prolamin is then separated through a subsequent alcohol-based precipitation step, ensuring complete removal of non-protein material. This method enables the efficient recovery of high-purity prolamin from rice, particularly in cases where traditional methods may compromise protein integrity.

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A novel method for extracting and purifying rice albumin and globulin proteins from rice grains. The method leverages a unique combination of solvents and pH conditions to selectively separate and concentrate these proteins while minimizing interactions with other components. The process enables the efficient extraction of these hypoallergenic proteins, which are particularly valuable for infant formula and other food products, through a simpler and more controlled extraction process compared to traditional methods.

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Enzymatic hydrolysis method for preparing rice protein that addresses traditional extraction challenges. The method employs a continuous flow process using enzymes to selectively extract protein from rice residue, eliminating the need for traditional solvent extraction methods. The process involves continuous flow through a reactor containing enzymes that selectively hydrolyze the rice residue, with the resulting liquid containing the desired protein. The extracted liquid is then dried to produce a high-quality protein powder.

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A method for extracting natural antioxidant activity from rice protein through enzymatic hydrolysis, eliminating the need for acid and alkali processing and traditional enzymatic methods. The process involves hydrolyzing brown rice or rice bran protein using sodium chloride and ethanol at controlled temperatures, resulting in a purified protein extract that retains its antioxidant properties. This method provides a cost-effective, environmentally friendly alternative to traditional protein extraction methods.

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A method for extracting high-quality protein from rice protein isolate through a novel processing approach that utilizes rice slag as a valuable feedstock. The method involves converting rice slag into a sheet-like material through hammer crushing and subsequent expansion, which is then coated with the rice protein. This sheet material is then subjected to a series of physical separation steps, including centrifugation and filtration, to achieve the desired protein concentration. The resulting protein product exhibits superior nutritional quality compared to conventional methods, with enhanced protein efficiency ratio (PER) and bioavailability. The process not only converts waste materials into valuable protein but also reduces cellulase requirements and improves environmental sustainability.

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An integrated process for recovering ethanol and proteins from distiller's wet grain (DWG) obtained from a rice distillery. The process involves milling broken rice, partial hydrolysis, saccharification, fermentation, distillation, and separation of DWG into wet cake and filtrate. The wet cake is washed and separated into protein concentrate and liquid, which can be further treated with alkali or proteases to obtain purified proteins.

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A method to improve the solubility and functional properties of glutinous rice protein for potential food applications. The method involves two steps: (1) Extracting the protein from glutinous rice using alkali and acid precipitation. (2) Modifying the extracted protein using frozen ball milling and enzymatic hydrolysis. The ball milling step reduces particle size and improves solubility. Enzymatic hydrolysis further reduces particle size and improves solubility. The combined treatment also enhances antioxidant properties.

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Production system for rice protein and its by-products through a standardized process that combines rice processing, enzyme treatment, separation, and post-treatment steps. The system begins with rice processing, followed by enzyme treatment to liquefy the rice milk and starch, followed by separation to obtain a protein gel. The gel is then processed through saccharification and fermentation to produce a fermented syrup. The syrup undergoes further processing, including drying, to produce the final product. This system enables the production of high-quality rice protein while simultaneously generating valuable by-products through optimized processing steps.

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A large-scale process to purify proteins like RuBisCO from plant sources in a way that allows high yields, low costs, and produces pure, clean protein powders suitable for human and animal consumption. The process involves extracting the proteins by lysing the plant tissues, then using activated charcoal to adsorb undesirable pigments and impurities. The charcoal is separated out, leaving behind the purified protein solution. Further filtration steps can further isolate the protein. This provides a clean, pure, tasteless, odorless, colorless protein powder suitable for food applications.

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A method for expressing and purifying recombinant reteplase using genetically engineered rice as a bioreactor. The method involves constructing a plant expression vector containing a rice codon-optimized reteplase gene, transforming rice seeds with the vector, extracting a crude extract containing recombinant reteplase, and purifying the protein through a series of chromatography steps.

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Isolating organic plant proteins with high digestibility, balanced amino acids, and without chemical residues. The method involves grinding raw plant material, raising the pH of the resulting solution, separating solids, lowering the pH of the supernatant, precipitating, and drying to isolate the protein. This provides a clean, organic plant protein source without added sodium or chloride. The isolated proteins have improved digestibility, complete amino acid profiles, and are organic.

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A quantitative method for measuring protein content in rice using liquid chromatography-mass spectrometry (LC/MS). The method involves labeling a specific protein fragment with a stable isotope-labeled peptide, followed by LC/MS analysis to quantify the labeled peptide. This approach enables precise quantification of protein content in rice by selectively targeting specific protein fragments, eliminating interference from other proteins.

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High-purity rice protein powder that achieves superior protein quality and texture through a novel combination of rice processing and enzymatic hydrolysis. The process involves first extracting rice protein from rice residue using a combination of crushing, liquefaction, and spray drying. The extracted rice residue is then subjected to enzymatic hydrolysis to break down cellulose and other complex compounds. The resulting rice residue is then processed to achieve complete protein structure opening and complete starch removal, followed by surfactant-assisted separation of fat and other colloids. The final product undergoes spray drying and subsequent processing steps to achieve the desired particle size and texture.

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Rice protein isolate supplement that enhances exercise performance and recovery by providing optimal amino acid profiles for muscle growth and maintenance. The supplement contains less than 90 mg of leucine per gram and less than 50 mg of lysine per gram, making it an ideal choice for athletes seeking to improve muscle hypertrophy and endurance gains. The supplement also incorporates organic sprouted whole grain brown rice syrup solids, organic flavor, organic guar gum, organic gum arabic, organic stevia, sea salt, and organic sunflower oil.

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A method for purifying recombinant human fibronectin from genetically engineered rice seeds using a two-step chromatography process. The method involves extracting a crude protein mixture from the rice seeds, followed by cation exchange chromatography to obtain a primary product, and then anion exchange chromatography to obtain purified recombinant human fibronectin.

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Low-grainy rice protein powder with improved solubility and taste for food applications. The powder is made by enzymatic hydrolysis of rice protein with specific conditions to break down the protein structure without excessive hydrolysis that leads to bitterness and umami flavors. The steps involve pre-treating the rice dregs, controlled enzymatic hydrolysis, micro-jetting to reduce particle size, spray drying, and crushing. The powder has >80% protein, <3% fat, >30% nitrogen solubility, and <5% moisture with >70% protein below 2000 Da molecular weight.

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A process for isolating proteins from plant materials containing high levels of oils and fats, such as oilseeds and legumes, to produce protein isolates suitable for human consumption. The process involves a mild extraction step using an aqueous solvent, followed by a sequence of solvent exchanges using GRAS organic solvents to remove impurities and achieve high protein purity. The resulting protein isolates contain at least 70% native plant-based protein, less than 1% carbohydrates, and minimal levels of phenolic compounds and other impurities.

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A method for identifying differential proteins in rice quality using high-throughput proteomics. The approach employs strong cation exchange columns to separate and purify proteins from rice samples, followed by mass spectrometry analysis. The method leverages a validated proteomics platform to identify and quantify protein variations associated with rice quality characteristics. These identified proteins are then verified through quantitative PCR (qPCR) to establish their functional significance. The method enables rapid identification of key proteins related to rice quality traits, providing a foundation for research into breeding high-quality rice varieties and developing transgenic lines.

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Enzyme-assisted method for simultaneous production of rice starch and protein through a single enzymatic process, enabling higher yields compared to traditional sequential methods. The process employs a single enzyme system that hydrolyzes both proteins and starch simultaneously, reducing the need for separate enzyme treatments and resulting in improved efficiency and cost-effectiveness.

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A rice protein powder production process that enhances the yield of rice starch while reducing processing costs. The process involves using an isotropic machine to separate rice protein from rice starch through a single-step process, followed by a centrifugal separation to produce a high-quality rice protein powder. The isotropic machine combines the properties of both centrifugal and ultrasonic processing to efficiently separate the starch and protein components, resulting in a starch polysaccharide with a value of 1.75.

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