Research on Lactobacillus Casei Probiotic for Gut Health

Lactic acid bacteria (LAB) are the main probiotics currently available in the markets and are essential for maintaining gut health. To guarantee probiotic function, it is imperative to boost the culture yield of probiotic organisms, ensure the sufficient viable cells in commercial products, or develop effective prebiotics. Recent studies have shown that protein hydrolysates and their derived peptides promote the proliferation of probiotic

Source

The genus Lactobacillus is a heterogeneous group of lactic acid bacteria (LAB) with important implications in biotechnology. It is a predominant microorganism in the world of gastrointestinal health, but various other uses are being explored. They have long been considered in the generally recognized as safe (GRAS) category by the Food and Drug Authority (FDA). They have been extensively used in fermentation and there is growing interest regarding their use in gut health, vaccine production, and biomedical innovation. This chapter highlights the application of lactobacilli in healthcare.

Source

A method for screening personalized probiotics, prebiotics, foods, health functional foods, and drugs using fecal samples and a proprietary medium composition. The method involves treating fecal samples with candidate materials, culturing, and analyzing the resulting microbiota and metabolites to identify effective personalized microbiota-improving agents. The composition includes L-cysteine, sodium chloride, sodium carbonate, potassium chloride, and hemin, which are combined with fecal samples to create a controlled in vitro environment that mimics the human gut. The method enables rapid screening of personalized microbiota-improving agents using fecal samples and special media, and can be used to diagnose diseases caused by intestinal disorders.

Source

Using microbiome compositions to modulate metabolites in a subject, like humans, for therapeutic purposes. The compositions contain specific bacterial strains that can alter metabolite levels in the subject's body. By administering these microbiome compositions, it allows targeting specific metabolites associated with diseases or conditions. The compositions can be used to treat diseases or prevent conditions by modulating the subject's metabolome. The strains can be identified by comparing metabolite levels in samples before and after administration to find strains that significantly change metabolite levels.

Source

Probiotics, such as

Source

Abstract The gut microbiota harbors a complex ecosystem of bacteria that govern host health homeostasis. Alterations to the intestinal environment, known as gut dysbiosis, is associated with several diseases. Targeting the gut with microbiome-engineered therapeutics, such as probiotics, is a promising approach to restore microbial homeostasis and host health. Probiotics can effectively improve the gut environment, although strain-specific mechanisms remain largely unknown. Thus, this study aims to identify beneficial action of select probiotics to modulate the gut environment through antioxidative and anti-inflammatory properties. To this end, we tested the interaction among three probiotic strains Lactobacillus gasseri A237 (LgA237), Lactobacillus plan-tarum WCFS1 (LpWCFS1) and Lactobacillus fermentum NCIMB 5221 (Lf5221) and a human intestinal epithelial cell line, HT-29, for adhesion properties, radical scavenging abilities and anti-inflammatory activities. All three probiotics adhere well to HT-29 cells, indicating proper gut colonization. LpWCFS1 demonstrated the greatest adh... Read More

Source

Probiotics play diverse roles in promoting health and well-being, encompassing gut microbiota balance, digestive health, immune function, gut barrier integrity, metabolic health, and mental well-being. With the beneficial effects of probiotics, individuals may improve their gastrointestinal health, boost immune function, and reduce the risk of various diseases. These probiotics compete with pathogens and support gut and immune homeostasis to help in the prevention and cure of gastrointestinal disease, immune disorders, diabetes, obesity, cancer and allergic disorders, etc. Probiotic-rich foods and supplements into the diet offer a proactive approach to maintaining overall health and optimizing the functioning of the gut. Probiotics help to maintain immune homeostasis and decrease the risk of inflammatory disorders and allergic conditions. Several probiotic strains are available on the market and are being consumed in the form of foods, supplements, or drugs for health benefits. Lactobacilli, Bifidobacteria, and Bacillus species are common probiotic strains.

Source

Lactic acid bacteria have a long and extensive application record as probiotic strains. Lately, due to wide-spread use of molecular-genetic, transcirptomic, proteomic, metabolomic etc. studies and the massive accumulation of data on the structure and functions of symbiotic intestinal microbiota, the interest in probiotic microorganisms tends to expand year-by-year. Lactic acid bacteria were the first used probiotic species, are still of sharp market demand and have been recognized as the most thoroughly studied microbes among functional relatives. However, characteristics inherent to probiotic bacteria are strain-specific, as a rule, and for preparations with defined purpose it is essential to select special strains showing a complex of appropriate properties. It accentuates the top relevance of research aimed at selection of lactic acid bacterial strains suitable for diverse probiotic preparations. For several decades we curried out investigations to isolate strains of lactic acid bacteria from various natural sources, to characterize their properties and to derive technologies of p... Read More

Source

Multi-biotic dietary supplements that combine a postbiotic or postbiotic precursor, a probiotic blend, and a prebiotic blend in a single dose. The supplements can be formulated with an inner capsule containing the probiotic and prebiotic blend, and an outer capsule containing the postbiotic or postbiotic precursor. The inner and outer capsules can have different dissolution or degradation rates to optimize delivery of each component to the gastrointestinal tract. The supplements can be designed to improve gut health, digestive health, and immune health by providing a synergistic combination of postbiotics, probiotics, and prebiotics.

Source

Probiotic Lactobacillus strains that improve food component digestibility through enhanced nutritional metabolism. The strains, isolated from dairy products, fruits, and vegetables, exhibit superior digestive capabilities compared to conventional probiotics. The strains achieve this through enhanced hydrolysis of proteins, phenolic compounds, and raffinose, as well as increased bioavailability of these nutrients. The strains are formulated as a dietary supplement for oral administration, with a carrier that maintains their viability and activity.

Source

The gut microbiota is the central regulator of human metabolism, it is considered a superorganism, it fulfills functions related to homeostasis and the maintenance of systemic health, contributing to immunity and epithelial integrity, any alteration is related to non-compliance or interruption of some of its functions, for this reason in search of balance, prevention and treatment measures have been implemented, one of them is the use of probiotics, which has been proven to bring beneficial actions towards the health of the host. , such as lactobacillus and bifidobacteria, here we will review the association between gut microbiota, probiotics and the use of probiotics like a treatment of prevention for pathologies of gut microbiota.

Source

A novel use of Lactobacillus paracasei subsp. paracasei K56 for enhancing intestinal immunity and resistance to bacterial infection. The strain significantly reduces pathogenic bacterial adhesion to intestinal epithelial cells, improves barrier function, and activates intestinal immunity. It can be used in food, pharmaceutical, or feed products to prevent diarrhea and combat bacterial infections.

Source

The probiotics, when administered in adequate amounts, have the ability to 
 confer health benefits to the host by positively influencing the composition and 
 function of gut microbiota. The present study was aimed to isolate and 
 characterize the probiotic bacteria from locally available curd and Yakult 
 samples and compare them with Sporolac for probiotic properties. Three 
 bacterial isolates were isolated and tentatively identified on morpho-cultural and 
 biochemical characteristics as Lactobacillus sp. The isolates were named 
 Lactobacillus sp. isolates PBC, PBY and SPO. E coli was used as negative control. 
 The study revealed that the isolates exhibited auto-aggregation and co 
 aggregation activities, more in isolate SPO as compared to the isolates PBC and 
 PBY. Biofilm and quorum quenching activities were high in Lactobacillus sp. 
 isolate PBC and PBY as compared to Lactobacillus sp. isolate SPO. The other 
 tests showed more or less similar properties as compared to the commercially 
 available product.... Read More

Source

Agents for stimulating the growth of bifidobacteria in the mammalian gut, comprising Lactobacillus fermentum cells, Lactobacillus delbrueckii cells, or a combination of both, as well as their culture medium, which can be administered in various forms including LACTEOL, low-lactose LACTEOL, or supernatant fractions. These agents can be used to improve gut health by increasing bifidobacteria populations, which has been linked to various beneficial effects including reduced inflammation, improved metabolic function, and enhanced immune response.

Source

Background: Probiotics are different types of friendly types of yeasts or bacteria that improve the health of humans as well as animals. Their formulation can be used to make a wide range of products, including foods, pharmaceuticals, nutritional supplements and animal feed. Numerous scientific studies have examined and demonstrated the benefits of probiotics for health. Since there may be concerns regarding the safety of probiotics, a thorough understanding of their advantages and disadvantages is crucial before they are widely used. The aim of this study is to shed light on the probiotic world, which has a long history of success across many different fields. Main Body: The definition of probiotics and related terminology, their usage, and the kinds of bacterial strains involved as different species of Lactobacillus; Bifidobacterium. etc., their mode of action and their applications for humans and animals were all covered in this study. Numerous advantages for human health were highlighted, including their ability to treat a range of gastrointestinal issues and their role in loweri... Read More

Source

A growing number of probiotic-containing products are on the market, and their use is increasing. Probiotics are thought to support the health of the gut microbiota, which in turn might prevent or delay the onset of gastrointestinal tract disorders. Obesity, type 2 diabetes, autism, osteoporosis, and some immunological illnesses are among the conditions that have been shown to possibly benefit from probiotics. In addition to their ability to favorably affect diseases, probiotics represent a defense system enhancing intestinal, nutritional, and oral health. Depending on the type of microbial strain utilized, probiotics can have variable beneficial properties. Although many microbial species are available, the most widely employed ones are lactic acid bacteria and bifidobacteria. The usefulness of these bacteria is dependent on both their origin and their capacity to promote health. Probiotics represent a valuable clinical tool supporting gastrointestinal health, immune system function, and metabolic balance. When used appropriately, probiotics may provide benefits such as a reduced ri... Read More

Source

Abstract: This study explores the potential of using probiotic lactic acid bacteria (LAB) that produce bacteriocins as a novel approach to controlling gut microbiota dysbiosis. Gut dysbiosis, characterized by an imbalance in the composition of the gut microbiota, is implicated in various health disorders. Probiotic LAB have gained attention for their ability to promote a balanced gut microbiota. Bacteriocin-producing LAB offer an added advantage by inhibiting pathogenic bacteria and promoting the growth of beneficial microbes. This review summarizes the current knowledge on the mechanisms of probiotic LAB and their bacteriocins in modulating gut microbiota composition. Additionally, key clinical studies and potential applications of bacteriocin-producing LAB in mitigating gut dysbiosis are discussed.

Source

Abstract BACKGROUND Lactocaseibacillus paracasei subsp. paracasei NTU 101 (NTU101) is a wellknown commercial probiotic with multiple health beneficial effects. In this study, the gut microbiota modulation effect of an NTU 101 product, Vigiis 101LAB, on healthy human was investigated in a randomized, doubleblind, placebocontrolled human trial. RESULTS Vigiis 101LAB significantly modulated human gut microbiota at fourth and sixth weeks of trial (anosim analysis, P = 0.001). It also significantly improved peristalsis ( P = 0.003) and shortened defecation interval of subjects. The shift of gut microbiota is significantly fit with defecation interval ( P = 0.009) and stool shape ( P = 0.001) of subjects. CONCLUSION Our results suggest that Vigiis 101LAB promotes human intestinal health with improvement of peristalsis and fecal quality. The gut modulation effects of Vigiis 101LAB subsequently raised the abundance of vitamin B7, vitamin K, pyrimidine and purine biosynthesis pathways. Vigiis 101LAB may promote peristalsis via purinergic pathway and possibly conferring prophylactic be... Read More

Source

Probiotics are known for their beneficial effects on improving intestinal function by alleviating the gut microbial diversity.

Source

In recent years, interest in probiotics, particularly Lactobacillus strains like Lactobacillus sp. SKG34, isolated from Sumbawa mare milk, has surged due to their potential health benefits. This strain holds promise as a probiotic agent, but understanding its behavior in the gastrointestinal (GI) tract is essential for effective application. This study aimed to investigate the resistance and persistence of Lactobacillus sp. SKG34 in the GI tract of rats. Rats were orally administered the strain at a concentration of 10^8 cells for 30 days. The probiotics ability to modulate gut microbiota and improve host health is a key area of interest. By utilizing techniques like PCR-RAPD, this research sought to elucidate the fate of Lactobacillus sp. SKG34 in the complex environment of the rat GI tract. Results revealed a significant increase in LAB population in cecal contents, from 3.06 x 10^9 cfu/g in the control group to 1.25 x 10^10 cfu/g in the probiotic group. RAPD analysis also detected Lactobacillus sp. SKG34 in approximately 23% of the total population of lactic acid bacteria in ceca... Read More

Source