Formulations for Epigenetic Modulation in Skin Aging

Umbilical cord mesenchymal stem cells (UC-MSCs) with enhanced proliferation and resistance to skin aging. The stem cells are modified to overexpress miR-626. This increases their proliferation capacity in vitro. The UC-MSC exosomes secreted by the miR-626 overexpressing cells also have enhanced anti-aging activity when applied to skin cells. The miR-626 overexpression and exosome secretion can be used to develop improved stem cell therapies and cosmeceuticals for skin rejuvenation.

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Anti-aging strategy targeting epigenetics through exosomes derived from embryonic stem cells. The approach involves processing replicative aging mouse cells with exosomes secreted by embryonic stem cells, which selectively target epigenetic alterations associated with aging. By delivering specific epigenetic modifications to aging cells, the exosomes induce reversible epigenetic reprogramming that counteracts the aging process. This therapeutic approach leverages the unique properties of embryonic stem cell-derived exosomes to selectively target and reverse epigenetic changes characteristic of aging cells.

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Umbilical cord mesenchymal stem cell exosomes with enhanced miR-328-3p expression promote skin barrier repair through improved proliferation and matrix composition. These exosomes, derived from umbilical cord mesenchymal stem cells, exhibit increased expression of miR-328-3p, a microRNA involved in cell differentiation and proliferation. The exosomes enhance dermal fibroblast proliferation and matrix production, particularly in the context of skin aging, by regulating key collagen and elastin genes. This exosomal delivery system has potential applications in anti-aging skin care products and therapies.

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Therapeutic agents that modulate ELOVL2 expression and fatty acid metabolism to delay or reverse aging. The agents target the ELOVL2 enzyme, which is crucial for polyunsaturated fatty acid synthesis, and enhance its activity. By increasing ELOVL2 expression, these agents promote cellular energy metabolism, reduce inflammation, and slow cellular aging processes. The therapeutic agents can be formulated as compositions of polyunsaturated omega-3 and omega-6 fatty acids, which are known to support ELOVL2 activity.

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A method and composition for anti-aging that targets senescent cells through the inhibition of key regulatory proteins. The method employs a small RNA molecule (miR-302) that specifically targets and suppresses SA-β-Gal and P16 proteins, while promoting H3K9me3 protein and type III collagen COL3A1 expression. This approach enables the suppression of senescent cell-related phenotypes without inducing pluripotent stem cell-like reprogramming or disrupting normal cellular functions. The miR-302 molecule can be delivered through non-therapeutic means, such as transfection or viral vectors, and has been shown to effectively reverse senescence in various cell types.

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Modulating cell age through epigenetic reprogramming to achieve a youthful transcriptional and epigenetic profile. The method employs CRISPR-Cas9 genome editing to target specific epigenetic markers associated with aging, specifically those involved in cellular aging signatures. By selectively modifying these markers, the system can restore a youthful epigenetic profile in differentiated cells, effectively "reversing" the aging process. This approach enables the reprogramming of cells from old donors to a youthful state, with potential therapeutic applications in regenerative medicine.

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