Formulations for MicroRNA-Mediated Skin Rejuvenation

A miRNA from Xylella schizophora that regulates the expression of microtubule-associated protein 2 (Mical2) and its downstream skin aging markers. The miRNA, miR-CM1, targets Mical2 and modulates downstream skin aging pathways, including collagen synthesis and cell senescence. This miRNA has potential applications in anti-aging skin care products, particularly in formulations containing emulsions like emulsion containing H1-CM1, which exhibits enhanced skin protection against UV-induced aging.

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Using kale extract as a natural ingredient to boost skin health by increasing expression of the anti-inflammatory microRNA miR-146 in skin cells. Kale extract was found to upregulate miR-146 levels in human skin cells, leading to reduced inflammation, oxidative stress, and apoptosis. The extract also increased collagen and hyaluronic acid production, and inhibited NO and cytokine release. This suggests kale extract can have moisturizing, anti-wrinkle, antioxidant, and repairing skin loss effects by modulating miR-146 levels.

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A recombinant miRNA combination for sensitive skin repair that targets multiple pathways of skin barrier damage. The combination of miR-138-5p and miR-129-5p specifically targets the keratinocyte migration and proliferation pathways, while also modulating inflammatory responses. By combining these miRNAs, the therapeutic effect is enhanced compared to targeting individual miRNAs alone, with improved efficacy in accelerating skin barrier repair and reducing inflammation.

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A novel approach to skin anti-aging through the use of microRNA (miRNA) in the preparation of anti-aging products. The approach involves the upregulation of specific miRNAs, including miR146A, which targets genes involved in skin aging and wrinkle formation. These miRNAs regulate the expression of key collagen, elastin, and laminin genes, while inhibiting matrix metalloproteinases that degrade these components. The miRNA-mRNA interaction pathway leads to enhanced collagen synthesis and reduced degradation, resulting in improved skin elasticity and wrinkle prevention. The miRNA-mRNA interaction pathway is verified through quantitative PCR analysis of skin fibroblast cells. This novel approach provides a new mechanism for anti-aging products to address skin aging through targeted miRNA regulation of critical skin components.

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MicroRNA (miRNA) enhances skin repair and whitening through targeted regulation of cellular processes. The miRNA, specifically miR146A, promotes the clearance of apoptotic cells at tissue damage sites, thereby accelerating the repair of the skin barrier. By up-regulating key skin barrier genes, miR146A enables the efficient regeneration of the skin's protective barrier function. This novel approach enables the development of effective skin repair and whitening products that target the underlying mechanisms of skin damage and regeneration.

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A method for preventing and treating photoaging through the delivery of miR-660-5p, a microRNA that regulates fibroblast aging and skin damage. The method involves delivering miR-660-5p into exosomes secreted by fibroblasts, which then transport the miRNA to target cells. This approach enables targeted delivery of miR-660-5p to fibroblasts, where it can prevent or reverse fibroblast aging and skin damage through regulation of key fibroblast genes.

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A mesodermal anti-aging exosome mixture containing exosomes derived from fibroblasts and exosomes derived from mesenchymal stem cells. The exosome mixture is prepared by culturing fibroblasts and mesenchymal stem cells separately, harvesting and purifying the exosomes from their culture media, and mixing them in a 1:1 volume ratio to obtain the anti-aging exosome mixture. The fibroblast-derived exosomes promote collagen synthesis, elastin production, and extracellular matrix remodeling for skin firmness and elasticity. Mesenchymal stem cell-derived exosomes modulate immune responses, promote angiogenesis, and secrete growth factors for skin rejuvenation and aging reduction. The exosome mixture contains at least 2*10^9 exosomes

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Screening active substances for improving skin photoaging through the identification of skin photoaging targets. The method involves analyzing skin microRNA expression profiles in normal skin samples exposed to UV radiation and protected from UV radiation, followed by identifying specific microRNAs associated with skin aging. These microRNAs are then used to develop novel cosmetic actives that target their respective pathways, providing a novel approach to skin rejuvenation.

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Recombinant miRNA with enhanced skin repair properties, particularly for sensitive skin conditions. The miRNA, anti-miR-138p, is engineered to selectively target and regulate the expression of genes involved in skin barrier integrity and keratinocyte function. This miRNA promotes keratinocyte proliferation, migration, and barrier repair, while inhibiting inflammatory responses. The miRNA's therapeutic effects are demonstrated in skin barrier repair models, particularly in 3D epidermal injury models, where it enhances stratum corneum thickness and improves skin barrier function.

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A biological product for mammalian skin and/or hair follicle anti-aging that uses exosomes extracted from the blood of young animals to combat aging. The exosomes contain specific microRNAs like miR-144, miR-149, and miR-455. The exosomes are extracted from young animal blood using ultracentrifugation and kits. The young animal exosomes are injected into old animals to slow down aging and improve skin/hair health. The exosomes replace or activate aging exosomes in old animals, improving cellular functions to prevent aging. The products can be used in anti-aging skincare and haircare products.

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A recombinant miRNA with therapeutic effects on skin barrier function and skin repair, particularly in the treatment of sensitive skin conditions. The miRNA, miR-23a-3p, is engineered to target specific skin barrier-related genes, such as filaggrin, involucrin, and keratinocyte shedding enzymes, to restore skin integrity. The miRNA is produced through recombinant expression in bacteria, offering a cost-effective and scalable alternative to traditional RNA synthesis methods. Its therapeutic efficacy is demonstrated through in vitro and in vivo studies, including skin barrier repair assays and keratinocyte migration and proliferation tests.

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A therapeutic approach for preventing skin premature aging through the targeted regulation of skin fibroblast function. The therapy employs miR-582-5p, a microRNA that has been implicated in various cellular processes, including fibroblast proliferation and collagen synthesis. By modulating miR-582-5p expression, the therapy promotes the proliferation of skin fibroblasts while inhibiting the expression of aging markers, thereby enhancing collagen production and delaying skin aging.

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Umbilical cord mesenchymal stem cell exosomes with enhanced miR-328-3p expression promote skin barrier repair through improved proliferation and matrix composition. These exosomes, derived from umbilical cord mesenchymal stem cells, exhibit increased expression of miR-328-3p, a microRNA involved in cell differentiation and proliferation. The exosomes enhance dermal fibroblast proliferation and matrix production, particularly in the context of skin aging, by regulating key collagen and elastin genes. This exosomal delivery system has potential applications in anti-aging skin care products and therapies.

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A recombinant anti-miR-129-5p designed to improve skin aging by enhancing fibroblast activity and collagen synthesis. The recombinant anti-miR-129-5p is engineered to be expressed in skin fibroblasts through a tRNA scaffold, and its expression is specifically targeted to senescent skin cells. This recombinant anti-miR-129-5p promotes collagen production and fibroblast proliferation while inhibiting senescent marker genes, thereby reducing skin aging indicators. The recombinant anti-miR-129-5p can be formulated into cosmetic products, including creams, lotions, and masks, to deliver its anti-aging benefits.

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A method for developing a cosmetic active ingredient that improves skin aging. The method involves screening a microRNA marker of skin endogenous aging and using it to develop a cosmetic active ingredient that targets the marker. The marker is identified by comparing microRNA expression profiles of young and old skin samples. The marker is then used to develop a cosmetic active ingredient that targets the marker and improves skin aging.

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Exosomes derived from human umbilical cord mesenchymal stem cells (UC-MSCs) that selectively enhance skin regeneration by modulating dermal fibroblast function. These exosomes, engineered to express miR-328-3p, specifically target and up-regulate genes involved in fibroblast proliferation and collagen production, while simultaneously inhibiting fibroblast degradation. This targeted approach enables the delivery of therapeutic benefits to the skin through exosomes, with potential applications in anti-aging treatments.

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Formulation of topical compositions and methods to enhance Mir-146a expression in skin cells, with a focus on promoting PER1 gene expression, DNA repair, and stem cellularity. The compositions contain a Mir-146a activator and an adjuvant, which together stimulate Mir-146a expression in skin cells. The activator targets specific pathways to enhance PER1 expression and DNA repair, while the adjuvant enhances the activator's effectiveness. The compositions are used to treat skin conditions by restoring circadian rhythms, repairing DNA damage, and promoting stem cell health.

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A method and composition for anti-aging that targets senescent cells through the inhibition of key regulatory proteins. The method employs a small RNA molecule (miR-302) that specifically targets and suppresses SA-β-Gal and P16 proteins, while promoting H3K9me3 protein and type III collagen COL3A1 expression. This approach enables the suppression of senescent cell-related phenotypes without inducing pluripotent stem cell-like reprogramming or disrupting normal cellular functions. The miR-302 molecule can be delivered through non-therapeutic means, such as transfection or viral vectors, and has been shown to effectively reverse senescence in various cell types.

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A method for identifying and characterizing skin aging markers through high-throughput microRNA profiling. The method involves comparing the expression profiles of skin microRNAs in normal skin versus skin exposed to UV radiation and air pollution. By analyzing the differential expression of specific microRNAs, researchers can identify key regulators of skin aging, including those involved in epidermal junction integrity, stress response, and cell proliferation. These microRNAs can serve as biomarkers for identifying effective anti-aging treatments by targeting specific pathways involved in skin aging.

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A topical agent that enhances skin renewal by regulating key skin aging genes. The agent is derived from the ethanol extract of Magnolia sativus and demonstrates potent anti-aging effects through its ability to up-regulate extracellular matrix genes, basement membrane-related genes, and anti-aging-related genes. This agent stabilizes skin structure, regulates elastic fiber synthesis, and prevents skin aging by promoting cell proliferation, differentiation, and migration.

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